Isolation and Characterization of Innate Lymphoid Cells within the Murine Tumor Microenvironment.

Isolation and Characterization of Innate Lymphoid Cells within the Murine Tumor Microenvironment.
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小鼠肿瘤微环境中先天淋巴细胞的分离和表征。

DOI:
10.1007/978-1-0716-0338-3_14
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发表时间:
2020
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
通讯作者:
Mallett G
Mallett G
中科院分区:
--
文献类型:
--
作者:
Mallett G

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先天淋巴样细胞(ILCs)对组织免疫和组织动态平衡都具有重要作用。它们被分成三组:第一组ILCs包括NK细胞,它们在诱导对细胞内病原体的免疫中起重要作用;第二组ILCs保护细胞免受寄生虫的侵袭;第三组ILCs保护细胞免受细胞外病原体的攻击。ILC在癌症免疫中的作用尚不清楚。在本章中,我们讨论了在实验性B16黑色素瘤小鼠模型中分离和鉴定肿瘤微环境中肿瘤浸润性ILC亚群的方法。本章还强调了PD-1在肿瘤微环境中各种ILC亚群上的表达。
Innate lymphoid cells (ILCs) are important for both tissue immunity and tissue homeostasis. They are classified into three groups: Group 1 ILCs include NK cells, which are important in eliciting immunity against intracellular pathogens; group 2 ILCs protect against parasitic helminths; and group 3 ILCs protect against extracellular pathogens. The role of ILCs in cancer immunity remains unclear. In this chapter, we discuss methods for isolating and characterizing tumor-infiltrating ILC subsets within the tumor microenvironment in an experimental murine model of B16 melanoma. The chapter also highlights the expression of PD-1 on the various ILC subsets within the tumor microenvironment.
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