Transgenic manipulation of triacylglycerol biosynthetic enzymes in B. napus alters lipid-associated gene expression and lipid metabolism.
Transgenic manipulation of triacylglycerol biosynthetic enzymes in B. napus alters lipid-associated gene expression and lipid metabolism.
复制标题
DOI：
10.1038/s41598-022-07387-x
复制
发表时间：
2022-03-01
影响因子：
4.6
通讯作者：
Scofield S
中科院分区：
文献类型：
--
作者：
Liao P;Lechon T;Romsdahl T;Woodfield H;Fenyk S;Fawcett T;Wallington E;Bates RE;Chye ML;Chapman KD;Harwood JL;Scofield S
油菜（Brassica napus）是一种重要的作物，其栽培是为了在其种子中产生油（主要是三酰甘油; TAG）。TAG合成主要由肯尼迪途径中的关键酶控制，例如甘油3-磷酸酰基转移酶（GPAT）、溶血磷脂酸酰基转移酶（LPAT）和二酰基甘油酰基转移酶（DGAT），但也可以通过酶的活性由磷酸甘油酯（例如磷脂酰胆碱（PC））产生：二酰基甘油酰基转移酶（PDAT）。为了评估这些酶改变油产量或组成的潜力，我们分析了转基因B。使用来自拟南芥和旱芹的异源转基因过表达GPAT、LPAT或PDAT的油菜系，并检查了与WT相比这些系中的脂质谱和基因表达的变化。PC和TAG丰度和空间分布在胚胎组织中的明显变化，观察到在一些转基因系，以及改变表达的基因一般参与酰基脂质代谢。总体而言，我们的研究结果表明，这些关键酶的上调差异影响脂质组成和分布以及脂质相关基因的表达，提供了重要的信息，可用于通过代谢工程来改善作物特性。
Oilseed rape (Brassica napus) is an important crop that is cultivated for the oil (mainly triacylglycerol; TAG) it produces in its seeds. TAG synthesis is controlled mainly by key enzymes in the Kennedy pathway, such as glycerol 3-phosphate acyltransferase (GPAT), lysophosphatidate acyltransferase (LPAT) and diacylglycerol acyltransferase (DGAT) but can also be produced from phosphoglycerides such as phosphatidylcholine (PC) by the activity of the enzyme phospholipid: diacylglycerol acyltransferase (PDAT). To evaluate the potential for these enzymes to alter oil yields or composition, we analysed transgenic B. napus lines which overexpressed GPAT, LPAT or PDAT using heterologous transgenes from Arabidopsis and Nasturtium and examined lipid profiles and changes in gene expression in these lines compared to WT. Distinct changes in PC and TAG abundance and spatial distribution in embryonic tissues were observed in some of the transgenic lines, together with altered expression of genes involved generally in acyl-lipid metabolism. Overall our results show that up-regulation of these key enzymes differentially affects lipid composition and distribution as well as lipid-associated gene expression, providing important information which could be used to improve crop properties by metabolic engineering.
登录
查看更多内容
影响因子：
7.4
作者：
Li, Fengling;Wu, Xuemin;Kunst, Ljerka
通讯作者：
Kunst, Ljerka
影响因子：
5.6
作者：
Li Y;Qi B
通讯作者：
Qi B
DOI：
10.1016/j.bbalip.2016.03.021
发表时间：
2016-09-01
影响因子：
4.8
作者：
Bates, Philip D.
通讯作者：
Bates, Philip D.
影响因子：
11.6
作者：
Greer, Michael Scott;Cai, Yingqi;Chapman, Kent D.
通讯作者：
Chapman, Kent D.
DOI：
10.1073/pnas.120067297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Dahlqvist, A;St책hl, U;Stymne, H
通讯作者：
Stymne, H