Identification and characterization of a novel locus in Cytophaga hutchinsonii involved in colony spreading and cellulose digestion
Identification and characterization of a novel locus in Cytophaga hutchinsonii involved in colony spreading and cellulose digestion
复制标题
哈钦森噬细胞菌中参与菌落扩散和纤维素消化的新位点的鉴定和表征
DOI：
10.1007/s00253-015-6412-9
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发表时间：
2015-02
期刊：
影响因子：
--
通讯作者：
Wrifeng Liu
中科院分区：
文献类型：
--
作者：
Tengteng Yang;Weixin Zhang;Guanjun Chen;Wrifeng Liu
Hutchinsonii是一种好氧降解土壤纤维素的细菌，能够快速降解结晶纤维素并在地表快速滑行。然而，所涉及的机制在很大程度上是未知的。在这里，我们使用基于水手的转座子HimarEm1来筛选缺乏利用滤纸作为唯一碳源的Hutchinsonii突变体。发现了一个编码假想蛋白的新基因位点chu_1719，该基因的失活导致了华支睾吸虫在滤纸上的生长受阻。进一步的分析表明，CHU_1719的破坏抑制了菌落的扩散，但对Avicel在液体介质中的降解没有显著影响。突变体膜蛋白的羧甲基纤维素酶(CMCase)活性较野生型降低约40%。此外，突变株和野生型(WT)菌株的纤维素吸附外膜蛋白图谱也有显著差异。这些结果表明，CHU_(1719)可能通过影响华支睾吸虫细胞膜蛋白的适当产生而在控制其扩散运动和纤维素利用方面发挥重要作用。
Cytophaga hutchinsonii, an aerobic cellulolytic soil bacterium, is capable of degrading crystalline cellulose and gliding over surface rapidly. The involved mechanisms, however, are largely unknown. Here, we used the mariner-based transposon HimarEm1 to screen for C. hutchinsonii mutants deficient in utilizing filter paper as the sole carbon source. A novel gene locus, chu_1719, encoding a hypothetical protein was identified, whose inactivation resulted in a compromised growth of C. hutchinsonii on filter paper. Further analysis revealed that disruption of chu_1719 suppressed colony spreading but had no significant effect on Avicel degradation in liquid medium. Carboxymethylcellulase (CMCase) activity of the mutant membrane proteins was reduced by about 40% as compared with the wild-type strain. Moreover, profiles of cellulose-adsorbed outer membrane proteins were significantly different between the mutant and wild-type (WT) strains. These results suggest that chu_1719 plays an important role in controlling the spreading motility and cellulose utilization probably by affecting the appropriate production of membrane proteins in C. hutchinsonii.
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影响因子：
3.2
作者：
M. Feldhaus;V. Hwa;Q. Cheng;A. Salyers
通讯作者：
M. Feldhaus;V. Hwa;Q. Cheng;A. Salyers
DOI：
10.1007/978-3-642-56968-5_4
发表时间：
2000
期刊：
Comput. Oper. Res.
影响因子：
--
作者：
S. Surzycki
通讯作者：
S. Surzycki
影响因子：
3.2
作者：
Rhodes, Ryan G.;Samarasam, Mudiarasan Napoleon;McBride, Mark J.
通讯作者：
McBride, Mark J.
影响因子：
3.2
作者：
Rhodes, Ryan G.;Nelson, Shawn S.;McBride, Mark J.
通讯作者：
McBride, Mark J.
影响因子：
3.2
作者：
Stanier, RY
通讯作者：
Stanier, RY