[10]-Gingerdiols as the major metabolites of [10]-gingerol in zebrafish embryos and in humans and their hematopoietic effects in zebrafish embryos.
[10]-Gingerdiols as the major metabolites of [10]-gingerol in zebrafish embryos and in humans and their hematopoietic effects in zebrafish embryos.
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DOI：
10.1021/jf401501s
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发表时间：
2013-06-05
影响因子：
6.1
通讯作者：
Sang S
中科院分区：
文献类型：
--
作者：
Chen H;Soroka DN;Haider J;Ferri-Lagneau KF;Leung T;Sang S
姜酚是新鲜生姜中的一系列主要成分，其中含量最丰富的是[6]-、[8]-和[10]-姜酚(6G、8G和10G)。我们之前发现，生姜提取物及其纯化成分，特别是10G，潜在地刺激斑马鱼胚胎的原始和决定性的造血波(血细胞形成)。然而，目前仍不清楚10G的代谢物是否保持了母体化合物对病理性贫血的治疗效果。在本研究中，我们首先研究了10G在斑马鱼胚胎中的代谢，然后探索了10G在人类体内的生物转化。我们的结果表明，10G在斑马鱼胚胎和人类体内都有广泛的代谢，通过对MSN光谱的分析和与我们合成的标准物质的比较，鉴定了其中的两个主要代谢物：(3S，5S)-[10]-姜二醇和(3R，5S)-[10]-姜二醇。斑马鱼胚胎处理24小时后，10G大部分转化为其代谢物。我们的结果清楚地表明，还原途径是10G在斑马鱼胚胎和人类中的主要代谢途径。此外，我们还研究了10G及其两种代谢产物在斑马鱼胚胎中的造血作用，它们具有与10G相似的造血作用。
Gingerols are a series of major constituents in fresh ginger with the most abundant being [6]-, [8]-, and [10]-gingerols (6G, 8G, and 10G). We previously found that ginger extract and its purified components, especially 10G, potentially stimulate both the primitive and definitive waves of hematopoiesis (blood cell formation) in zebrafish embryos. However, it is still unclear if the metabolites of 10G retain the efficacy of the parent compound towards pathological anemia treatment. In the present study, we first investigated the metabolism of 10G in zebrafish embryos, and then explored the biotransformation of 10G in humans. Our results show that 10G was extensively metabolized in both zebrafish embryos and in humans, in which two major metabolites, (3S,5S)-[10]-gingerdiol and (3R,5S)-[10]-gingerdiol, were identified by analysis of the MSn spectra and comparison to authentic standards that we synthesized. After 24 hours of treatment of zebrafish embryos, 10G was mostly converted to its metabolites. Our results clearly indicate the reductive pathway is a major metabolic route for 10G in both zebrafish embryos and in humans. Furthermore, we investigated the hematopoietic effect of 10G and its two metabolites, which show similar hematopoietic effects as 10G in zebrafish embryos.
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影响因子：
3.7
作者：
Ferri-Lagneau KF;Moshal KS;Grimes M;Zahora B;Lv L;Sang S;Leung T
通讯作者：
Leung T
DOI：
10.1158/1055-9965.epi-07-2934
发表时间：
2008-08
期刊：
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology
影响因子：
--
作者：
Zick SM;Djuric Z;Ruffin MT;Litzinger AJ;Normolle DP;Alrawi S;Feng MR;Brenner DE
通讯作者：
Brenner DE
影响因子：
3.9
作者：
Chen, Huadong;Lv, Lishuang;Sang, Shengmin
通讯作者：
Sang, Shengmin
影响因子：
2
作者：
Jiang, HL;S贸lyom, AM;Gang, DR
通讯作者：
Gang, DR
影响因子：
3.8
作者：
KIKUZAKI, H;TSAI, SM;NAKATANI, N
通讯作者：
NAKATANI, N