Four novel cellulose synthase (CESA) genes from Birch (Betula platyphylla Suk.) involved in primary and secondary cell Wall biosynthesis.
Four novel cellulose synthase (CESA) genes from Birch (Betula platyphylla Suk.) involved in primary and secondary cell Wall biosynthesis.
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来自桦树（Betula platyphylla Suk.）的四种新型纤维素合酶（CESA）基因参与初级和次级细胞壁生物合成
DOI：
10.3390/ijms131012195
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发表时间：
2012-09-25
影响因子：
5.6
通讯作者：
Yang C
中科院分区：
文献类型：
--
作者：
Liu X;Wang Q;Chen P;Song F;Guan M;Jin L;Wang Y;Yang C
纤维素合酶（CESA）是植物细胞壁生物质生成的重要催化剂，主要由包含十个或更多成员的CesA基因家族编码。在这项研究中;采用RT-PCR结合RACE方法，从重要用材树种白桦中分离得到编码CESA的4个全长cDNA，分别命名为BplCesA3、-4、-7和-8。这些推导的 CESA 包含与其拟南芥同源物相同的典型结构域和区域。这四个基因的 cDNA 长度不同，从推导的氨基酸序列推断出的各个蛋白质结构域的位置也不同，其氨基酸序列同一性仅为 63.8% 至 70.5%。实时 RT-PCR 显示所有四种 BplCesAs 在不同组织中均以不同水平表达。结果表明BplCESA8可能参与次生细胞壁生物合成和花发育。 BplCESA3 以独特的表达模式出现，可能参与初生细胞壁生物合成和种子发育；它也可能与同型半乳糖醛酸的合成有关。 BplCESA7和BplCESA4可能与纤维素合酶复合物的形成有关，主要参与次生细胞壁生物合成。成熟花粉中四种 BplCESA 的表达丰度极低，表明它们很少参与桦木成熟花粉的形成。四种 BplCesAs 的独特表达模式表明它们可能参与各种组织的发育，并且它们可能受到桦木中不同机制的控制。
Cellulose synthase (CESA), which is an essential catalyst for the generation of plant cell wall biomass, is mainly encoded by the CesA gene family that contains ten or more members. In this study; four full-length cDNAs encoding CESA were isolated from Betula platyphylla Suk., which is an important timber species, using RT-PCR combined with the RACE method and were named as BplCesA3, −4, −7 and −8. These deduced CESAs contained the same typical domains and regions as their Arabidopsis homologs. The cDNA lengths differed among these four genes, as did the locations of the various protein domains inferred from the deduced amino acid sequences, which shared amino acid sequence identities ranging from only 63.8% to 70.5%. Real-time RT-PCR showed that all four BplCesAs were expressed at different levels in diverse tissues. Results indicated that BplCESA8 might be involved in secondary cell wall biosynthesis and floral development. BplCESA3 appeared in a unique expression pattern and was possibly involved in primary cell wall biosynthesis and seed development; it might also be related to the homogalacturonan synthesis. BplCESA7 and BplCESA4 may be related to the formation of a cellulose synthase complex and participate mainly in secondary cell wall biosynthesis. The extremely low expression abundance of the four BplCESAs in mature pollen suggested very little involvement of them in mature pollen formation in Betula. The distinct expression pattern of the four BplCesAs suggested they might participate in developments of various tissues and that they are possibly controlled by distinct mechanisms in Betula.
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影响因子：
4
作者：
Liang, X;Joshi, CP
通讯作者：
Joshi, CP
DOI：
10.1073/pnas.0706569104
发表时间：
2007-09-25
影响因子：
11.1
作者：
Desprez, Thierry;Juraniec, Michal;Vernhettes, Samantha
通讯作者：
Vernhettes, Samantha
影响因子：
11.6
作者：
Kimura, S;Laosinchai, W;Brown, RM
通讯作者：
Brown, RM
影响因子：
11.6
作者：
Brown, DM;Zeef, LAH;Turner, SR
通讯作者：
Turner, SR
影响因子：
7.4
作者：
Mendu, Venugopal;Griffiths, Jonathan S.;DeBolt, Seth
通讯作者：
DeBolt, Seth