Multimodal magnetic-resonance/optical-imaging contrast agent sensitive to NADH.
Multimodal magnetic-resonance/optical-imaging contrast agent sensitive to NADH.
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DOI：
10.1002/anie.200900984
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发表时间：
2009
影响因子：
16.6
通讯作者：
Louie, Angelique Y.
中科院分区：
文献类型：
--
作者：
Tu, Chuqiao;Nagao, Ryan;Louie, Angelique Y.
Disruption of redox homoeostasis may lead to oxidative stress, that is, production of reactive oxygen species (ROS), and can induce many pathological conditions, including atherosclerosis, stroke, Alzheimer’s disease, Parkinson’s disease, and cancer.[1] Non-invasive observation of intracellular redox activities and their relationship to physiological function is a challenge for molecular imaging.[2] Magnetic resonance imaging (MRI) has recently emerged as a most promising tool for molecular imaging.[3] Typically MRI is not capable of sensing biochemical activity, but current advances in activatable contrast agents, which generate a signal in response to some variable in their immediate environment, hold the promise that MR contrast agents can be designed to be reporters of biological processes (ie pH, temperature, oxygen pressure, redox, enzyme, and metal-ion concentration).[3, 4] To date nitroxides are the most commonly used redox-sensitive paramagnetic contrast agents for MRI. However, the combination of relatively low relaxivity and short life spans limit the extensive use of MRI in observation of redox activities in living systems.[4c, 5]We have developed activatable MRI contrast agents by coupling a dye molecule, which undergoes remarkable structural change and charge shifts that are trigged by light or electrical activity,[6] to a macrocycle-based gadolinium complex, Gd (DO3A)(DO3A: 1, 4, 7, 10-tetraazacyclododecane-1, 4, 7-trisacetic acid). When an isomerization-inducing stimulation is applied, the structural change of the molecule influences the accessibility of water molecules to the GdIII center, causing the two isomers to have different magnetic properties, thus producing different contrast enhancement.[7] Herein, a spironaphthoxazine molecule which is from an established family of molecular switches was coupled to Gd (DO3A) to generate complex 1 (see scheme 1).[8] It was found that reduced nicotinamide adenine dinucleotide (NADH) can trigger the isomerization of spironaphthoxazine moiety in 1. The structural change resulted in a significant and immediate increase in MRI signal intensity accompanied by quenching of the intense fluorescence of 1.
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影响因子：
3.3
作者：
Magde, D;Rojas, GE;Seybold, PG
通讯作者：
Seybold, PG
影响因子：
6.3
作者：
Hyodo, Fuminori;Chuang, Kai-Hsiang;Krishna, Murali C.
通讯作者：
Krishna, Murali C.
影响因子：
2.4
作者：
Nadlinger, K;Birkmayer, J;Kunze, R
通讯作者：
Kunze, R
DOI：
10.1139/v83-054
发表时间：
1983-01-01
期刊：
CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE
影响因子：
--
作者：
CHU, NYC
通讯作者：
CHU, NYC
影响因子：
5.9
作者：
Forsyth, LM;Preuss, HG;Bellanti, JA
通讯作者：
Bellanti, JA