Distribution of capillary transit times in isolated lungs of oxygen-tolerant rats.
Distribution of capillary transit times in isolated lungs of oxygen-tolerant rats.
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DOI：
10.1007/s10439-010-0092-5
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发表时间：
2010-11
影响因子：
3.8
通讯作者：
Audi, Said H.
中科院分区：
文献类型：
--
作者：
Ramakrishna, Madhavi;Gan, Zhuohui;Clough, Anne V.;Molthen, Robert C.;Roerig, David L.;Audi, Said H.
关键词：
预先暴露于85%O2 5-7天的大鼠耐受100%O2的其他致死作用。目的是评价大鼠暴露于85%O2中7天对肺毛细血管平均通过时间（t_c）和毛细血管通过时间分布（hc（t））的影响。这一信息对于随后评价该高氧模型对肺毛细血管内皮细胞氧化还原代谢功能的影响是重要的。异硫氰酸荧光素标记右旋糖酐的静脉血药浓度-时间流出曲线（FITC-dex），一种血管内指示剂，和辅酶Q1氢醌（CoQ 1H 2），一种在通过肺循环时在血液和组织之间迅速平衡的化合物，在将其推注到暴露于室内空气的大鼠的离体灌注肺的肺动脉中后测量（常氧）或85%O2持续7天（高氧）。测定每个肺的FITC-dex和CoQ 1H 2流出曲线的矩（平均通过时间和方差），然后将其用于数学模型[Audi et al. J. Appl. Physiol. 77：332-351，1994]，以估计t Δ c和hc（t）的相对离散度（RDc）。数据分析显示，暴露于高氧可使肺tTc降低42%，并使RDc（hc（t）异质性指标）增加40%。
Rats pre-exposed to 85% O2 for 5–7 days tolerate the otherwise lethal effects of 100% O2. The objective was to evaluate the effect of rat exposure to 85% O2 for 7 days on lung capillary mean transit time (t̄c) and distribution of capillary transit times (hc(t)). This information is important for subsequent evaluation of the effect of this hyperoxia model on the redox metabolic functions of the pulmonary capillary endothelium. The venous concentration vs. time outflow curves of fluorescein isothiocyanate labeled dextran (FITC-dex), an intravascular indicator, and coenzyme Q1 hydroquinone (CoQ1H2), a compound which rapidly equilibrates between blood and tissue on passage through the pulmonary circulation, were measured following their bolus injection into the pulmonary artery of isolated perfused lungs from rats exposed to room air (normoxic) or 85% O2 for 7 days (hyperoxic). The moments (mean transit time and variance) of the measured FITC-dex and CoQ1H2 outflow curves were determined for each lung, and were then used in a mathematical model [Audi et al. J. Appl. Physiol. 77: 332–351, 1994] to estimate t̄c and the relative dispersion (RDc) of hc(t). Data analysis reveals that exposure to hyperoxia decreases lung t̄c by 42% and increases RDc, a measure hc(t) heterogeneity, by 40%.
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DOI：
10.1152/ajplung.00064.2005
发表时间：
2005-11-01
影响因子：
4.9
作者：
Audi, SH;Bongard, RD;Merker, MP
通讯作者：
Merker, MP
影响因子：
3.9
作者：
CADENAS, E;BOVERIS, A;STOPPANI, AOM
通讯作者：
STOPPANI, AOM
影响因子：
3.8
作者：
Audi, SH;Linehan, JH;Dawson, CA
通讯作者：
Dawson, CA
影响因子：
--
作者：
FRANK, L;IQBAL, J;MASSARO, D
通讯作者：
MASSARO, D
DOI：
10.1152/ajplung.00185.2003
发表时间：
2003-11-01
影响因子：
4.9
作者：
Audi, SH;Bongard, RD;Merker, MP
通讯作者：
Merker, MP