Antioxidant activity and protective effects of Tripterygium regelii extract on hydrogen peroxide-induced injury in human dopaminergic cells, SH-SY5Y.
Antioxidant activity and protective effects of Tripterygium regelii extract on hydrogen peroxide-induced injury in human dopaminergic cells, SH-SY5Y.
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抗氧化活性和三墨晶提取物对人多巴胺能细胞中过氧化氢损伤的保护作用，SH-SY5Y。
DOI：
10.1007/s11064-010-0185-4
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发表时间：
2010-08
影响因子：
4.4
通讯作者：
Kim SJ
中科院分区：
文献类型：
--
作者：
Choi BS;Sapkota K;Kim S;Lee HJ;Choi HS;Kim SJ
研究雷公藤提取物(TrE)对过氧化氢(H_2O_2)诱导的人多巴胺能细胞SH-SY5Y的抗氧化作用和神经保护作用。Tre含有相当多的酚类物质(282.73 mg单宁酸当量/克提取物)和类黄酮类化合物(101.43 mg柚皮苷当量/克提取物)。其还原能力和清除DPPH自由基的IC50值分别为52.51和47.83μg。Tre清除H_2O_2的能力为57.68μM×μg−1min−_1。通过检测Tre对H_2O_2损伤的SH-SY5Y细胞的影响，发现Tre在H_2O_2暴露前与细胞孵育后，H_2O_2诱导的细胞毒性被显著逆转，细胞形态改变、核固缩和DNA片段化等细胞凋亡特征被抑制。此外，TrE对H_2O_2诱导的线粒体膜电位的破坏和细胞的凋亡也有很好的抑制作用。Tre提取液能有效抑制Bax、Caspase-3和-9的表达上调，以及Bcl2的表达下调。此外，Tre可明显增加SH-SY5Y细胞酪氨酸羟化酶(TH)和脑源性神经营养因子(BDNF)的表达。这些发现表明，TrE保护SH-SY5Y细胞免受H_2O_2诱导的损伤，抗氧化特性可能是其神经保护作用的原因，并提示Tre可能作为一种预防与氧化应激相关的神经退行性疾病的药物。
The present work was conducted to investigate the antioxidant activity and neuroprotective effects of Tripterygium regelii extract (TRE) on H2O2-induced apoptosis in human dopaminergic cells, SH-SY5Y. TRE possessed considerable amounts of phenolics (282.73 mg tannic acid equivalents/g of extract) and flavonoids (101.43 mg naringin equivalents/g of extract). IC50 values for reducing power and DPPH radical scavenging activity were 52.51 and 47.83 μg, respectively. The H2O2 scavenging capacity of TRE was found to be 57.68 μM × μg−1 min−1. By examining the effects of TRE on SH-SY5Y cells injured by H2O2, we found that after incubation of cells with TRE prior to H2O2 exposure, the H2O2 induced cytotoxicity was significantly reversed and the apoptotic features such as change in cellular morphology, nuclear condensation and DNA fragmentation was inhibited. Moreover, TRE was very effective attenuating the disruption of mitochondrial membrane potential and apoptotic cell death induced by H2O2. TRE extract effectively suppressed the up-regulation of Bax, Caspase-3 and -9, and down-regulation of Bcl-2. Moreover, TRE pretreatment evidently increased the tyrosine hydroxylase (TH) and brain-derived neurotrophic factor (BDNF) in SH-SY5Y cells. These findings demonstrate that TRE protects SH-SY5Y cells against H2O2-induced injury and antioxidant properties may account for its neuroprotective actions and suggest that TRE might potentially serve as an agent for prevention of neurodegenerative disease associated with oxidative stress.
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影响因子：
7.4
作者：
Barbouti, A;Doulias, PT;Galaris, D
通讯作者：
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影响因子：
5.1
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7.4
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4.4
作者：
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6.1
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