Nitrosylated proteins in monocytes as a new marker of oxidative-nitrosative stress in diabetic subjects with macroangiopathy.
Nitrosylated proteins in monocytes as a new marker of oxidative-nitrosative stress in diabetic subjects with macroangiopathy.
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单核细胞中的亚硝基化蛋白是糖尿病患者氧化硝化应激的新标志，患有大型疾病。
DOI：
10.1055/s-2008-1078710
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发表时间：
2009-02
期刊：
影响因子：
--
通讯作者：
Obrosova IG
中科院分区：
文献类型：
--
作者：
Julius U;Drel VR;Grässler J;Obrosova IG
过氧亚硝酸盐在糖尿病并发症的发病机制中起重要作用。外周血单核细胞中的亚硝基化蛋白表达反映了细胞内过氧亚硝酸盐损伤，因此可能是比血浆硝基酪氨酸水平更高的诊断和预后价值的标志物。本初步研究的目的是评估糖尿病受试者的外周血单核细胞是否积累亚硝基化蛋白，以及亚硝基化蛋白表达是否与血糖控制、血脂谱变量、C反应蛋白浓度（炎症标志物）相关，以及是否在有和无糖尿病大血管和微血管并发症的患者中存在差异。评估了31名糖尿病受试者（29名2型糖尿病受试者，2名1型糖尿病受试者; 20名男性，11名女性;平均年龄66岁）外周血单核细胞中的亚硝基化蛋白表达（蛋白质印迹分析）。微血管病变的存在定义为视网膜病变、白蛋白排泄和/或神经病变，大血管病变定义为颈动脉斑块、心肌梗死和/或卒中病史。糖尿病受试者在外周血单核细胞中积累了大量的亚硝基化蛋白。在整个队列以及糖尿病大血管病变受试者中，亚硝基化蛋白表达与体重、血糖、HbA 1C和血浆C反应蛋白浓度呈正相关。单核细胞亚硝基化蛋白表达是糖尿病大血管病变患者代谢控制和炎症的新生物标志物。需要在更大的患者群体中对糖尿病微血管并发症进行更详细的评估，以确定该变量是否可以用作糖尿病神经病变、视网膜病变和肾病的存在、严重程度和进展的生物标志物。
Peroxynitrite plays an important role in the pathogenesis of diabetic complications. Nitrosylated protein expression in peripheral blood monocytes reflects intracellular peroxynitrite injury, and thus could be a marker of higher diagnostic and prognostic value than plasma nitrotyrosine level. The purpose of this pilot study was to assess if peripheral blood monocytes of diabetic subjects accumulate nitrosylated proteins, and if nitrosylated protein expression correlates with blood glucose control, variables of lipid profile, C-reactive protein concentration (a marker of inflammation), and differs in patients with and without diabetic macrovascular and microvascular complications. Nitrosylated protein expression in peripheral blood monocytes (Western blot analysis) was assessed in 31 subjects with diabetes mellitus (29 Type 2, 2 Type 1; 20 males, 11 females; mean age 66 years). The presence of microangiopathy was defined by retinopathy, albumin excretion, and/or neuropathy, and macroangiopathy by carotid plaques, a history of myocardial infarction, and/or stroke. Diabetic subjects accumulated significant amounts of nitrosylated proteins in peripheral blood monocytes. Nitrosylated protein expression positively correlated with body weight, blood glucose, HbA1C, and plasma C-reactive protein concentrations in the whole cohort as well as in subjects with diabetic macroangiopathy. Monocyte nitrosylated protein expression is a new biomarker of metabolic control and inflammation in diabetic subjects with macroangiopathy. A more detailed assessment of diabetic microvascular complications in a larger group of patients is needed to determine if this variable can be employed as a biomarker of the presence, severity, and progression of diabetic neuropathy, retinopathy, and nephropathy.
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影响因子：
5
作者：
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通讯作者：
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影响因子：
3.2
作者：
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DOI：
10.1152/ajpheart.01297.2005
发表时间：
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影响因子：
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7.7
作者：
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