A labile phosphodiester bond at the ligation junction in a circular intervening sequence RNA.
A labile phosphodiester bond at the ligation junction in a circular intervening sequence RNA.
复制标题
环状间插序列 RNA 中连接处的不稳定磷酸二酯键。
作者：
A. Zaug;J. Kent;T. Cech
The excised intervening sequence of the Tetrahymena ribosomal RNA precursor mediates its own covalent cyclization in the absence of any protein. The circular molecule undergoes slow reopening at a single phosphodiester bond, the one that was formed during cyclization. The resulting linear molecule has 5'-phosphate and 3'-hydroxyl termini; these are unusual products for RNA hydrolysis but are typical of the other reactions mediated by this molecule. The reopened circle retains cleavage-ligation activity, as evidenced by its ability to undergo another round of cyclization and reopening. The finding that an RNA molecule can be folded so that a specific phosphate can be strained or activated helps to explain how the activation energy is lowered for RNA self-splicing. The proposed mechanisms may be relevant to several other RNA cleavage reactions that are RNA-mediated.
从四膜虫核糖体 RNA 前体中切除的插入序列含有一个不由 DNA 编码的 5 末端鸟苷残基。
影响因子：
14.9
作者：
Zaug,AJ;Cech,TR
通讯作者：
Cech,TR
影响因子：
2.9
作者：
BREHM, SL;CECH, TR
通讯作者：
CECH, TR