Morphological and molecular identification of arrhenotokous strain of Diglyphuswani (Hymenoptera, Eulophidae) found in China as a control agent against agromyzid leafminers.
Morphological and molecular identification of arrhenotokous strain of Diglyphuswani (Hymenoptera, Eulophidae) found in China as a control agent against agromyzid leafminers.
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DOI：
10.3897/zookeys.1071.72433
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发表时间：
2021
期刊：
影响因子：
1.3
通讯作者：
Liu WX
中科院分区：
文献类型：
--
作者：
Du SJ;Yefremova Z;Ye FY;Zhu CD;Guo JY;Liu WX
双线虫属物种对于潜叶蝇来说具有重要的生态和经济意义。 2018年，首次报道和描述了一种产雌孤雌生殖物种Diglyphuswani Liu、Zhu和Yefremova。随后，在中国的云南省和贵州省发现了产雄D.wani。我们比较了产雌产菌株和产雌菌株的形态特征。然而，两个品系的雌性具有非常相似的形态，并且在前翅和后翅上表现出细微的差异。所不同的是，产雌产雌前翅整体刚毛较密，表现为肋部背面有2～4行刚毛，基部刚毛15～21毛；产雌产雌前翅背面有2列刚毛，基部一般有10～15毛。产雌产雌性后翅边缘静脉下方的排列比产雌产雌性相同区域的密度更密。为了探索 D.wani 产雌产雌菌株和产雌孤雌菌株之间的遗传差异，应用了线粒体和核基因并进行了测序。多基因分析表明，可以通过 COI、ITS1 和 ITS2 区分两个菌株。两个菌株之间的平均序列差异分别为0.052、0.010和0.007。然而，28S基因是不可行的，因为它包含不同菌株之间共享的单倍型。两株D.wani是针对潜叶蝇的优势寄生蜂，这种有效的可识别基础为今后深入研究两株D.wani的生物学特性以及田间应用提供了依据。
Diglyphus species are ecologically and economically important on agromyzid leafminers. In 2018, a thelytokous species, Diglyphuswani Liu, Zhu & Yefremova, was firstly reported and described. Subsequently, the arrhenotokous D.wani were discovered in Yunnan and Guizhou Provinces of China. We compared the morphological characteristics of thelytokous and arrhenotokous strains. However, the females of two strains had a strongly similar morphology and showed subtle differences in fore- and hind-wings. The difference was that forewing of arrhenotokous female was with denser setae overall, showing that costal cell with 2 ~ 4 rows of setae on dorsal surface and the setae of basal cell with 15 ~ 21 hairs and forewing of thelytokous female was with two rows of setae on dorsal surface and basal cell with 10 ~ 15 hairs generally. The setation beneath the marginal vein of the hind-wing of arrhenotokous female is denser than the same area of thelytokous female. To explore the genetic divergence between thelytokous and arrhenotokous strains of D.wani, the mitochondrial and nuclear gene were applied and sequenced. The polygenic analyses revealed that two strains can be distinguished by COI, ITS1 and ITS2. The mean sequence divergence between the two strains was 0.052, 0.010 and 0.007, respectively. Nevertheless, the 28S gene was unfeasible due to its containing a sharing haplotype between different strains. The two strains of D.wani are dominant parasitoids against agromyzid leafminers and such effective discernible foundation provides future in-depth studies on biological characteristics, along with insight into field application of two strains of D.wani.
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影响因子：
4.1
作者：
Chen, Y;Hui, XA;Huang, DW
通讯作者：
Huang, DW
DOI：
10.1098/rsbl.2003.0025
发表时间：
2003-08-07
影响因子：
4.7
作者：
Hebert, PDN;Ratnasingham, S;deWaard, JR
通讯作者：
deWaard, JR
影响因子：
1.9
作者：
Bocher, Jens;Nachman, Gosta
通讯作者：
Nachman, Gosta
影响因子：
2.1
作者：
Adachi-Hagimori, Tetsuya;Miura, Kazuki;Abe, Yoshihisa
通讯作者：
Abe, Yoshihisa
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro