An Immune-Responsive Cytoskeletal-Plasma Membrane Feedback Loop in Plants.
An Immune-Responsive Cytoskeletal-Plasma Membrane Feedback Loop in Plants.
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DOI：
10.1016/j.cub.2018.05.014
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发表时间：
2018-07-09
期刊：
影响因子：
--
通讯作者：
Deeks MJ
中科院分区：
文献类型：
--
作者：
Sassmann S;Rodrigues C;Milne SW;Nenninger A;Allwood E;Littlejohn GR;Talbot NJ;Soeller C;Davies B;Hussey PJ;Deeks MJ
细胞壁附着是植物免疫系统通过抑制植物细胞生长来阻止微生物入侵的反应性产物。该过程需要质膜（PM）的受控内陷与屏障材料输出到植物PM和细胞壁之间的体积相协调。植物肌动蛋白动力学是必不可少的，这种反应，但目前还不清楚如何胞吐和细胞骨架连接在空间和时间，形成功能性的CWA。在这里，我们表明，肌动蛋白依赖的贩运到免疫反应位点的拟南芥提供膜整合FORMIN 4，这反过来又有助于当地的细胞骨架动力学。全内反射荧光（TIRF）显微镜结合FORMIN 4-GFP表达的受控诱导揭示了动态的囊泡体群，这些囊泡体通过肌动蛋白依赖性过程在PM处积累形成簇。失活的FORMIN 4及其密切的同源物部分损害随后的防御和改变丝状肌动蛋白（F-肌动蛋白）分布在成熟的CWA。FORMIN 4的定位是稳定的，并且与内体网络的动态运输分离。此外，FORMIN 4在PM处与PEN 3的介观结构域的镶嵌揭示了肌动蛋白依赖性免疫货物的目的地的精细空间分离。总之，我们的数据表明一个模型，其中FORMIN 4是一个空间反馈元件在一个多层的，时间上定义的序列的细胞骨架响应。这种位置反馈对动态CWA边界的肌动蛋白丝分布和入侵前防御的结果做出了重大贡献。在免疫应答过程中，FORMIN 4被递送到细胞壁强化位点。递送需要肌动蛋白丝，并导致肌动蛋白动力学的局部增强。FORMIN 4和近同源物的遗传破坏导致防御表型。表明宿主蛋白质运输到真菌病原体接触的离散位点包括一种特定的膜整合蛋白，其在免疫应答期间加强局部细胞骨架动力学。
Cell wall appositions (CWAs) are produced reactively by the plant immune system to arrest microbial invasion through the local inversion of plant cell growth. This process requires the controlled invagination of the plasma membrane (PM) in coordination with the export of barrier material to the volume between the plant PM and cell wall. Plant actin dynamics are essential to this response, but it remains unclear how exocytosis and the cytoskeleton are linked in space and time to form functional CWAs. Here, we show that actin-dependent trafficking to immune response sites of Arabidopsis thaliana delivers membrane-integrated FORMIN4, which in turn contributes to local cytoskeletal dynamics. Total internal reflection fluorescence (TIRF) microscopy combined with controlled induction of FORMIN4-GFP expression reveals a dynamic population of vesicular bodies that accumulate to form clusters at the PM through an actin-dependent process. Deactivation of FORMIN4 and its close homologs partially compromises subsequent defense and alters filamentous actin (F-actin) distribution at mature CWAs. The localization of FORMIN4 is stable and segregated from the dynamic traffic of the endosomal network. Moreover, the tessellation of FORMIN4 at the PM with meso-domains of PEN3 reveals a fine spatial segregation of destinations for actin-dependent immunity cargo. Together, our data suggest a model where FORMIN4 is a spatial feedback element in a multi-layered, temporally defined sequence of cytoskeletal response. This positional feedback makes a significant contribution to the distribution of actin filaments at the dynamic CWA boundary and to the outcomes of pre-invasion defense. FORMIN4 is delivered to sites of cell wall reinforcement during immune responses Delivery requires actin filaments and causes local enhancement of actin dynamics Genetic disruption of FORMIN4 and close homologs causes a defense phenotype Transport and localization of FORMIN4 is distinct from key defense protein PEN3 Sassmann et al. show that the trafficking of host proteins to discrete sites of fungal pathogen contact includes a specific membrane-integrated formin that reinforces local cytoskeletal dynamics during an immune response.
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影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
3.5
作者：
Jarosch, B;Jansen, M;Schaffrath, U
通讯作者：
Schaffrath, U
影响因子：
64.8
作者：
Collins, NC;Thordal-Christensen, H;Schulze-Lefert, P
通讯作者：
Schulze-Lefert, P
DOI：
10.1073/pnas.1003619107
发表时间：
2010-12-14
影响因子：
11.1
作者：
Humphry, Matt;Bednarek, Pawel;Panstruga, Ralph
通讯作者：
Panstruga, Ralph
影响因子：
16.6
作者：
Li, Jiejie;Henty-Ridilla, Jessica L.;Staiger, Benjamin H.;Day, Brad;Staiger, Christopher J.
通讯作者：
Staiger, Christopher J.