Capping protein integrates multiple MAMP signalling pathways to modulate actin dynamics during plant innate immunity.
Capping protein integrates multiple MAMP signalling pathways to modulate actin dynamics during plant innate immunity.
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DOI：
10.1038/ncomms8206
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发表时间：
2015-05-28
影响因子：
16.6
通讯作者：
Staiger, Christopher J.
中科院分区：
文献类型：
--
作者：
Li, Jiejie;Henty-Ridilla, Jessica L.;Staiger, Benjamin H.;Day, Brad;Staiger, Christopher J.
植物和动物通过模式识别受体感知不同的微生物相关分子模式（MAMP）并激活先天免疫信号传导。肌动蛋白细胞骨架已被认为是先天免疫信号传导的靶点和细胞反应的关键转导子。然而，肌动蛋白重塑的分子机制和这些重排在先天免疫过程中的确切功能在很大程度上仍然未知。在这里，我们展示了快速肌动蛋白重塑响应几个不同的MAMP信号通路在植物表皮细胞。肌动蛋白动力学的调节是基础防御机制的汇聚点，例如细胞壁强化和转录重编程。我们的肌动蛋白动力学和遗传研究的定量分析表明，MAMP刺激的肌动蛋白重塑是由于信号脂质，磷脂酸的加帽蛋白（CP）的抑制。此外，CP促进对细菌和真菌植物病原体的抗性。这些发现表明CP是植物先天免疫应答的中心靶标。 细胞骨架重塑是植物先天免疫反应的重要组成部分。在这里，Li等人证明病原体触发的肌动蛋白重塑是由于加帽蛋白（CP）的抑制，并表明CP是抵抗植物病原体所必需的。
Plants and animals perceive diverse microbe-associated molecular patterns (MAMPs) via pattern recognition receptors and activate innate immune signalling. The actin cytoskeleton has been suggested as a target for innate immune signalling and a key transducer of cellular responses. However, the molecular mechanisms underlying actin remodelling and the precise functions of these rearrangements during innate immunity remain largely unknown. Here we demonstrate rapid actin remodelling in response to several distinct MAMP signalling pathways in plant epidermal cells. The regulation of actin dynamics is a convergence point for basal defence machinery, such as cell wall fortification and transcriptional reprogramming. Our quantitative analyses of actin dynamics and genetic studies reveal that MAMP-stimulated actin remodelling is due to the inhibition of capping protein (CP) by the signalling lipid, phosphatidic acid. In addition, CP promotes resistance against bacterial and fungal phytopathogens. These findings demonstrate that CP is a central target for the plant innate immune response. Cytoskeletal remodelling is an important component of the innate immune response in plants. Here, Li et al. demonstrate that pathogen-triggered actin remodelling is due to the inhibition of capping protein (CP), and show that CP is required for resistance against plant pathogens.
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