Outer Membrane Vesicles Derived From Escherichia coli Regulate Neutrophil Migration by Induction of Endothelial IL-8.
Outer Membrane Vesicles Derived From Escherichia coli Regulate Neutrophil Migration by Induction of Endothelial IL-8.
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DOI：
10.3389/fmicb.2018.02268
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发表时间：
2018
影响因子：
5.2
通讯作者：
Gho YS
中科院分区：
文献类型：
--
作者：
Lee J;Yoon YJ;Kim JH;Dinh NTH;Go G;Tae S;Park KS;Park HT;Lee C;Roh TY;Di Vizio D;Gho YS
外膜囊泡（OMV）是由革兰氏阴性细菌（包括大肠杆菌）组成性释放的球形蛋白脂质纳米结构。虽然已经证明E.大肠杆菌OMV在体内刺激强烈的肺部炎症反应，中性粒细胞浸润到肺部，这是大肠杆菌OMV的机制。大肠杆菌OMV介导的中性粒细胞募集的特征不明显。在这项研究中，我们观察到中性粒细胞在体内向小鼠肺组织中的显著浸润，在腹腔内给予E. coli OMVs。此外，OMV和CD 31阳性内皮细胞共定位在小鼠肺中。体外实验结果表明，E. coli OMV能显著增加人微血管内皮细胞IL-8的释放，Toll样受体（TLR）4是识别大肠杆菌OMV的主要成分。coli OMV在人内皮细胞相关TLR中的表达。此外，在用E. coli OMVs。综上所述，我们的数据表明，E。coli OMV通过TLR 4和NF-κ B依赖性方式从内皮细胞释放IL-8/CXCL 1，有效地将中性粒细胞募集到肺中。
Outer membrane vesicles (OMVs) are spherical, proteolipid nanostructures that are constitutively released by Gram-negative bacteria including Escherichia coli. Although it has been shown that administration of E. coli OMVs stimulates a strong pulmonary inflammatory response with infiltration of neutrophils into the lungs in vivo, the mechanism of E. coli OMV-mediated neutrophil recruitment is poorly characterized. In this study, we observed significant infiltration of neutrophils into the mouse lung tissues in vivo, with increased expression of the neutrophil chemoattractant CXCL1, a murine functional homolog of human IL-8, on intraperitoneal administration of E. coli OMVs. In addition, OMVs and CD31-positive endothelial cells colocalized in the mouse lungs. Moreover, in vitro results showed that E. coli OMVs significantly increased IL-8 release from human microvascular endothelial cells and toll-like receptor (TLR)4 was found to be the main component for recognizing E. coli OMVs among human endothelial cell-associated TLRs. Furthermore, the transmigration of neutrophils was suppressed in the lung tissues obtained from TLR4 knockout mice treated with E. coli OMVs. Taken together, our data demonstrated that E. coli OMVs potently recruit neutrophils into the lung via the release of IL-8/CXCL1 from endothelial cells in TLR4- and NF-κB-dependent manners.
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影响因子：
3.1
作者：
Ismail, S;Hampton, MB;Keenan, JI
通讯作者：
Keenan, JI
影响因子：
15.9
作者：
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Kubes, P
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3.4
作者：
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Gho, Yong Song
影响因子：
56.9
作者：
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通讯作者：
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影响因子：
4.8
作者：
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通讯作者：
Kuehn, MJ