Mcm10 proteolysis initiates before the onset of M-phase.
Mcm10 proteolysis initiates before the onset of M-phase.
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DOI：
10.1186/1471-2121-11-84
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发表时间：
2010-10-28
期刊：
影响因子：
--
通讯作者：
Saxena S
中科院分区：
文献类型：
--
作者：
Kaur M;Sharma A;Khan M;Kar A;Saxena S
Mcm10 蛋白对于复制的起始和延伸阶段至关重要。人类细胞在有丝分裂过程中蛋白水解 Mcm10，大概是为了确保单轮复制。有人提出，后期促进复合物使 Mcm10 在 M 晚期和 G1 早期早期泛素化。与之前的工作相反，我们报告Mcm10的降解是在有丝分裂开始时开始的。免疫印迹和免疫荧光检测显示，Mcm10 水平在有丝分裂的所有阶段都很低。我们报告说，Mcm10 的降解不依赖于后期促进复合物。此外，M 期的蛋白水解可以由 Mcm10 的非重叠区域独立介导，显然采用冗余机制来确保下调。据信，有丝分裂期间 Mcm10 的蛋白水解是防止复制异常起始的重要机制，本研究描述了 Mcm10 在细胞周期这一阶段的调节。
Mcm10 protein is essential for initiation and elongation phases of replication. Human cells proteolyze Mcm10 during mitosis, presumably to ensure a single round of replication. It has been proposed that anaphase promoting complex ubiquitinates Mcm10 in late M and early G1 phases. In contrast to the previous work, we report that the degradation of Mcm10 is initiated at the onset of mitosis. Immunoblotting and immunofluorescence assays display that Mcm10 levels are low in all phases of mitosis. We report that Mcm10 degradation is not dependent on anaphase promoting complex. Further, the proteolysis in M-phase can be independently mediated by non-overlapping regions of Mcm10, apparently employing a redundant mechanism to ensure downregulation. It is believed that the proteolysis of Mcm10 during mitosis is a vital mechanism to prevent aberrant initiation of replication and the present study describes the regulation of Mcm10 during this phase of the cell-cycle.
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