High matrix metalloproteinase activity is a hallmark of periapical granulomas.
High matrix metalloproteinase activity is a hallmark of periapical granulomas.
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DOI：
10.1016/j.joen.2009.06.008
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发表时间：
2009-09
影响因子：
4.2
通讯作者：
Kapila, Yvonne Lorraine
中科院分区：
文献类型：
--
作者：
Garcia de Paula-Silva, Francisco Wanderley;D'Silva, Nishaj J.;Bezerra da Silva, Lea Assed;Kapila, Yvonne Lorraine
关键词：
在进行根管治疗之前无法区分根尖周囊肿和肉芽肿导致治疗结果的不确定性，因为囊肿的愈合率较低。在囊肿或肉芽肿内寻找分子的差异表达可以提供关于病变的身份的信息，或者提示可能形成未来治疗干预的基础的机制差异。因此，我们研究了肉芽肿和囊肿是否表现出细胞外基质（ECM）分子的差异表达。采用微阵列分析技术，研究人根尖肉芽肿、根尖囊肿和健康牙周膜组织中ECM分子的差异表达。由于基质金属蛋白酶（MMP）在微阵列分析中表现出最高的差异表达，MMP进一步研究了原位酶谱和免疫组化。使用单因素ANOVA随后Tukey检验分析数据。我们观察到囊肿和肉芽肿差异表达几种ECM分子，特别是那些来自基质金属蛋白酶（MMP）家族。与囊肿相比，肉芽肿在MMP-9染色区域表现出更高的MMP酶活性。这些区域由多形核细胞（PMNs）组成，与囊肿相反。同样，MMP-13在肉芽肿中表达的细胞数量多于囊肿。我们的研究结果表明，高酶MMP活性的中性粒细胞与MMP-9和MMP-13染色的细胞可能是肉芽肿的分子特征，不像根尖周囊肿。
Inability to distinguish periapical cysts from granulomas prior to performing root canal treatment leads to uncertainty in treatment outcomes, because cysts have lower healing rates. Searching for differential expression of molecules within cysts or granulomas could provide information with regard to the identity of the lesion or suggest mechanistic differences that may form the basis for future therapeutic intervention. Thus, we investigated whether granulomas and cysts exhibit differential expression of extracellular matrix (ECM) molecules. Human periapical granulomas, periapical cysts, and healthy periodontal ligament tissues were used to investigate the differential expression of ECM molecules by microarray analysis. Since matrix metalloproteinases (MMP) showed the highest differential expression in the microarray analysis, MMPs were further examined by in situ zymography and immunohistochemistry. Data were analyzed using one-way ANOVA followed by Tukey test. We observed that cysts and granulomas differentially expressed several ECM molecules, especially those from the matrix metalloproteinase (MMP) family. Compared to cysts, granulomas exhibited higher MMP enzymatic activity in areas stained for MMP-9. These areas were composed of polymorphonuclear cells (PMNs), in contrast to cysts. Similarly, MMP-13 was expressed by a greater number of cells in granulomas compared to cysts. Our findings indicate that high enzymatic MMP activity in PMNs together with MMP-9 and MMP-13 stained cells could be a molecular signature of granulomas, unlike periapical cysts.
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影响因子：
4.2
作者：
Schulz, Malte;von Arx, Thomas;Bosshardt, Dieter
通讯作者：
Bosshardt, Dieter
影响因子：
3.3
作者：
Hong, CY;Lin, SK;Wang, JS
通讯作者：
Wang, JS
DOI：
10.1111/j.1749-6632.1994.tb24719.x
发表时间：
1994-01-01
期刊：
INHIBITION OF MATRIX METALLOPROTEINASES: THERAPEUTIC POTENTIAL
影响因子：
--
作者：
KRANE, SM
通讯作者：
KRANE, SM
影响因子：
5
作者：
Liapatas, S;Nakou, M;Rontogianni, D
通讯作者：
Rontogianni, D
影响因子：
5
作者：
NAIR, PNR;SJOGREN, U;SUNDQVIST, G
通讯作者：
SUNDQVIST, G