The Brassica napus calcineurin B-Like 1/CBL-interacting protein kinase 6 (CBL1/CIPK6) component is involved in the plant response to abiotic stress and ABA signalling.
The Brassica napus calcineurin B-Like 1/CBL-interacting protein kinase 6 (CBL1/CIPK6) component is involved in the plant response to abiotic stress and ABA signalling.
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DOI：
10.1093/jxb/ers273
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发表时间：
2012-10
影响因子：
6.9
通讯作者：
Li XB
中科院分区：
文献类型：
--
作者：
Chen L;Ren F;Zhou L;Wang QQ;Zhong H;Li XB
从甘蓝型油菜中分离到一个CBL相互作用蛋白激酶(CIPK)基因BnCIPK6。通过酵母双杂交筛选，在甘蓝型油菜中鉴定出27个BnCIPK6的相互作用伙伴(包括BnCBL1)。BIFC进一步证实了BnCIPK6和BnCBL1在植物细胞中的相互作用。BnCIPK6和BnCBL1的表达受到盐和渗透胁迫、磷饥饿和脱落酸(ABA)的显著上调。此外，在盐、甘露醇和ABA处理下，BnCIPK6启动子活性在子叶和根中被强烈诱导。高表达BnCIPK6及其活化形式BnCIPK6M和BnCBL1的转基因拟南芥植株提高了对高盐低磷的耐受性，提示BnCBL1和BnCIPK6的功能相互作用可能在高盐缺磷信号转导途径中起重要作用。此外，BnCIPK6的激活使拟南芥植物对ABA高度敏感。另一方面，BnCIPK6在拟南芥cipk6突变体中的过度表达完全挽救了该突变体的低磷敏感和ABA不敏感的表型，进一步表明BnCIPK6参与了植物对高盐、缺磷和ABA信号的响应。
A CBL-interacting protein kinase (CIPK) gene, BnCIPK6, was isolated in Brassica napus. Through yeast two-hybrid screening, 27 interaction partners (including BnCBL1) of BnCIPK6 were identified in Brassica napus. Interaction of BnCIPK6 and BnCBL1 was further confirmed by BiFC (bimolecular fluorescence complementation) in plant cells. Expressions of BnCIPK6 and BnCBL1 were significantly up-regulated by salt and osmotic stresses, phosphorous starvation, and abscisic acid (ABA). Furthermore, BnCIPK6 promoter activity was intensively induced in cotyledons and roots under NaCl, mannitol, and ABA treatments. Transgenic Arabidopsis plants with over-expressing BnCIPK6, its activated form BnCIPK6M, and BnCBL1 enhanced high salinity and low phosphate tolerance, suggesting that the functional interaction of BnCBL1 and BnCIPK6 may be important for the high salinity and phosphorous deficiency signalling pathways. In addition, activation of BnCIPK6 confers Arabidopsis plants hypersensitive to ABA. On the other hand, over-expression of BnCIPK6 in Arabidopsis cipk6 mutant completely rescued the low-phosphate-sensitive and ABA-insensitive phenotypes of this mutant, further suggesting that BnCIPK6 is involved in the plant response to high-salinity, phosphorous deficiency, and ABA signalling.
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影响因子：
11.6
作者：
Chen, Yi-Fang;Li, Li-Qin;Wu, Wei-Hua
通讯作者：
Wu, Wei-Hua
影响因子：
11.6
作者：
Cheong, YH;Kim, KN;Luan, S
通讯作者：
Luan, S
影响因子：
7.2
作者：
D'Angelo, Cecilia;Weinl, Stefan;Kudla, Joerg
通讯作者：
Kudla, Joerg
影响因子：
7.4
作者：
Martinez-Atienza, Juliana;Jiang, Xingyu;Quintero, Francisco J.
通讯作者：
Quintero, Francisco J.
DOI：
10.1073/pnas.2034853100
发表时间：
2003-09-30
影响因子：
11.1
作者：
Ohta, M;Guo, Y;Zhu, JK
通讯作者：
Zhu, JK