High-fat diet induces obesity in adult mice but fails to develop pre-penile and penile vascular dysfunction.
High-fat diet induces obesity in adult mice but fails to develop pre-penile and penile vascular dysfunction.
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DOI：
10.1038/s41443-021-00440-9
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发表时间：
2022-04
影响因子：
2.6
通讯作者：
Hannan JL
中科院分区：
文献类型：
--
作者：
Odom MR;Hunt TC;Pak ES;Hannan JL
肥胖会导致心血管疾病、糖尿病和勃起功能障碍(ED)，从而降低整体生活质量。肥胖导致勃起功能障碍的机制尚不清楚。目前高脂饮食(HFD)诱导肥胖的小鼠模型产生了相互矛盾的结果。常见的“野生型”菌株之间的遗传变异可能解释了相互矛盾的数据。成年雄性C57BL/6N和6J小鼠饲喂45%高脂饲料12周。测量每周食物摄入量、体重增加和体脂百分比。12周后，测量主动脉、阴部内动脉和阴茎的体外血管反应性。我们评估了平滑肌的收缩能力、内皮依赖和非内皮依赖性的松弛以及阴茎神经递质介导的松弛。与C57BL/6J小鼠相比，C57BL/6N小鼠出现了更严重的肥胖和葡萄糖敏感性。饲喂HFD的两个品系的主动脉收缩减少，但HFD的阴部动脉和阴茎的收缩没有变化。有趣的是，在全身和阴茎血管系统中，内皮依赖性和非独立性的松弛都没有变化。同样，HFD不损害阴茎神经递质介导的松弛。喂养12周的HFD的两个菌株都出现了肥胖表型。然而，HFD并没有像以前的研究那样损害阴茎前或阴茎平滑肌的血管反应性，这表明这个临床前模型不能准确地代表肥胖诱导的ED的临床表型。
Obesity can lead to cardiovascular disease, diabetes, and erectile dysfunction (ED) which decreases overall quality of life. Mechanisms responsible for obesity induced ED are unknown. Current mouse models of high fat diet (HFD) induced obesity yield conflicting results. Genetic variants among common “wild type” strains may explain contradictory data. Adult male C57BL/6N and 6J mice were fed a 45% HFD for 12 weeks. Weekly food intake, weight gain, and body fat percentage were measured. After 12 weeks, ex vivo vascular reactivity was measured in aortas, internal pudendal arteries, and penises. We assessed smooth muscle contractility, endothelial-dependent and -independent relaxation, and penile neurotransmitter mediated relaxation. C57BL/6N mice developed greater obesity and glucose sensitivity compared to C57BL/6J mice. Aortas from both strains fed a HFD had decreased contraction, yet contraction was unchanged in HFD pudendal arteries and penises. Interestingly, endothelial-dependent and -independent relaxation was unchanged in both systemic and penile vasculature. Likewise, HFD did not impair penile neurotransmitter mediated relaxation. Both strains fed 12 weeks of HFD developed obese phenotypes. However, HFD did not impair pre-penile or penile smooth muscle vasoreactivity as demonstrated in previous studies, suggesting this preclinical model does not accurately represent the clinical phenotype of obesity induced ED.
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影响因子：
7.3
作者：
Xia N;Weisenburger S;Koch E;Burkart M;Reifenberg G;Förstermann U;Li H
通讯作者：
Li H
影响因子：
8.2
作者：
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Leiter, Edward H.
DOI：
10.1152/ajpheart.00027.2008
发表时间：
2008-05-01
影响因子：
4.8
作者：
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通讯作者：
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影响因子：
3.5
作者：
Hannan, Johanna L.;Blaser, Mark C.;Adams, Michael A.
通讯作者：
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