Tetrahymena: an alternative model host for evaluating virulence of Aeromonas strains.
Tetrahymena: an alternative model host for evaluating virulence of Aeromonas strains.
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DOI：
10.1371/journal.pone.0048922
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Liu YJ
中科院分区：
文献类型：
--
作者：
Pang MD;Lin XQ;Hu M;Li J;Lu CP;Liu YJ
一个简单的评价模型将有助于气单胞菌毒力的高通量筛选。先前的研究表明，四膜虫有可能成为检验嗜水气单胞菌毒力的容许模型。在这里，我们的目的是利用斑马鱼实验和四膜虫-气单胞菌共培养两种模型宿主来评估气单胞菌的毒力，并检查嗜热四膜虫模型的数据是否反映了已建立的动物模型中的感染情况。首先，测定39株气单胞菌在斑马鱼体内的50%致死量（LD50），评估其毒力。从1.3×102到3.0×107的LD50值表明这些菌株具有高到中等程度的毒力，可以用于评估四膜虫模型的毒力。在四膜单胞菌-气单胞菌共培养中，我们通过检测气单胞菌和四膜单胞菌的相对存活率来评估气单胞菌的毒力。当相对存活率大于60%时，分离气单胞菌被认为是有毒的，而如果相对存活率低于40%，分离气单胞菌被认为是无毒的。当嗜热t菌与气单胞菌共培养后的相对存活率低于40%时，认为该菌株具有毒性。相反，如果嗜热t菌的相对存活率大于50%，则该菌株被归类为无毒菌株。令人鼓舞的是，来自39个气单胞菌菌株的数据在斑马鱼和四膜虫-气单胞菌共培养模型中显示出良好的相关性。结果提供了足够的数据，证明四膜虫可以作为斑马鱼的可比替代品，用于测定气单胞菌分离株的毒力。
An easier assessment model would be helpful for high-throughput screening of Aeromonas virulence. The previous study indicated the potential of Tetrahymena as a permissive model to examine virulence of Aeromonas hydrophila. Here our aim was to assess virulence of Aeromonas spp. using two model hosts, a zebrafish assay and Tetrahymena-Aeromonas co-culture, and to examine whether data from the Tetrahymena thermophila model reflects infections in the well-established animal model. First, virulence of 39 Aeromonas strains was assessed by determining the 50% lethal dose (LD50) in zebrafish. LD50 values ranging from 1.3×102 to 3.0×107 indicated that these strains represent a high to moderate degree of virulence and could be useful to assess virulence in the Tetrahymena model. In Tetrahymena-Aeromonas co-culture, we evaluated the virulence of Aeromonas by detecting relative survival of Aeromonas and Tetrahymena. An Aeromonas isolate was considered virulent when its relative survival was greater than 60%, while the Aeromonas isolate was considered avirulent if its relative survival was below 40%. When relative survival of T. thermophila was lower than 40% after co-culture with an Aeromonas isolate, the bacterial strain was regarded as virulent. In contrast, the strain was classified as avirulent if relative survival of T. thermophila was greater than 50%. Encouragingly, data from the 39 Aeromonas strains showed good correlation in zebrafish and Tetrahymena-Aeromonas co-culture models. The results provide sufficient data to demonstrate that Tetrahymena can be a comparable alternative to zebrafish for determining the virulence of Aeromonas isolates.
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影响因子：
--
作者：
Jacobs, Mary Ellen;DeSouza, Leroi V.;Klobutcher, Lawrence A.
通讯作者：
Klobutcher, Lawrence A.
DOI：
10.1098/rspb.2007.0245
发表时间：
2007-08-22
影响因子：
4.7
作者：
Meltz Steinberg, Karyn;Levin, Bruce R.
通讯作者：
Levin, Bruce R.
影响因子：
5.4
作者：
Kingombe, CIB;Huys, G;Jemmi, T
通讯作者：
Jemmi, T
使用原生动物嗜热四膜虫开发嗜水气单胞菌感染模型
影响因子：
2.1
作者：
Li, Jing;Zhang, Xiao-Lu;Lu, Cheng-Ping
通讯作者：
Lu, Cheng-Ping
影响因子：
4.7
作者：
Rodriguez, I.;Novoa, B.;Figueras, A.
通讯作者：
Figueras, A.