Molecular mechanism by which the Notch signaling pathway regulates autophagy in a rat model of pulmonary fibrosis in pigeon breeder's lung.
Molecular mechanism by which the Notch signaling pathway regulates autophagy in a rat model of pulmonary fibrosis in pigeon breeder's lung.
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DOI：
10.1515/med-2023-0629
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发表时间：
2023
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本研究探讨了Notch信号通路和自噬参与鸽子肺(PBL)肺纤维化形成的分子机制。将大鼠分为对照组(Ctrl)、PBL模型组(M)、Notch信号抑制组(M+D)、自噬抑制组(M+W)和自噬诱导组(M+R)。以鸽皮冻干蛋白粉为变应原，建立PBL大鼠肝纤维化模型。通过抑制Notch通路和干扰自噬，探讨Notch信号在PBL肺纤维化中调节自噬的机制。M组和M+W组肺间质纤维化程度明显高于M+D组和M+R组。α-平滑肌肌动蛋白在M组、M+D组和M+W组的表达明显高于对照组(P<0.05)。细胞自噬标记物Beclin1和Lc3在M组、M+D组和M+W组的表达低于Ctrl组(P<0.05)，而Beclin1和Lc3在M+D组和M+R组的表达高于M组。M组、M+D组、M+W组和M+R组大鼠血清和肺组织中的活性氧水平均高于Ctrl组(P<0.05)。Notch信号通路通过调节自噬参与了PBL大鼠肺纤维化的病理过程。
This study investigated the molecular mechanisms underlying the involvement of the Notch signaling pathway and autophagy in the development of pulmonary fibrosis in pigeon breeder’s lung (PBL). Rats were divided into control (Ctrl), PBL model (M), M + D (Notch signaling inhibition), M + W (autophagy inhibition), and M + R (autophagy induction) groups. Lyophilized protein powder from pigeon shedding materials was used as an allergen to construct a fibrotic PBL rat model. The mechanism by which Notch signaling regulated autophagy in the pulmonary fibrosis of PBL was investigated by inhibiting the Notch pathway and interfering with autophagy. Pulmonary interstitial fibrosis was significantly greater in the M group and the M + W group than in the M + D and M + R groups. The expression of α-smooth muscle actin was significantly higher in the M, M + D, and M + W groups than in the Ctrl group (P < 0.05). The expression of the cell autophagy markers Beclin1 and LC3 was lower in the M, M + D, and M + W groups than in the Ctrl group (P < 0.05), whereas Beclin1 and LC3 expressions were higher in the M + D and M + R groups than in the M group. The levels of reactive oxygen species in serum and lung tissues were higher in the M, M + D, M + W, and M + R groups than in the Ctrl group (P < 0.05). The Notch signaling pathway is involved in the pathological process of pulmonary fibrosis in the rat model of PBL by regulating autophagy.
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DOI：
10.1038/nrneph.2016.54
发表时间：
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期刊：
Nature reviews. Nephrology
影响因子：
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期刊：
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影响因子：
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