Emergence and Rearrangement of Dynamic Supramolecular Aggregates Visualized by Interferometric Scattering Microscopy.
Emergence and Rearrangement of Dynamic Supramolecular Aggregates Visualized by Interferometric Scattering Microscopy.
复制标题
干涉散射显微镜观察动态超分子聚集体的出现和重排。
DOI：
10.1021/acsnano.0c02414
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发表时间：
2020-09-22
期刊：
影响因子：
17.1
通讯作者：
Fletcher SP
中科院分区：
文献类型：
--
作者：
Lebedeva MA;Palmieri E;Kukura P;Fletcher SP
研究自然环境中的动态自组装系统对于理解自组装的机制，从而对这些过程进行全面控制至关重要。传统的基于集成的分析方法往往难以揭示发生在单个粒子水平上的自组装的关键特征。在这里，我们描述了一种无标记的单粒子分析，通过干涉散射显微镜观察水溶液中的实时自组装。我们演示了该方法如何应用于产生胶束或囊泡聚集体的双相反应，检测聚集体形成的开始，量化单颗粒水平的动力学，并区分聚集体的s型和指数增长。此外，我们可以实时跟踪聚集体尺寸的演变，可视化自组装过程的成核阶段，并记录诸如油性组分进入胶束或囊泡腔等现象。
Studying dynamic self-assembling systems in their native environment is essential for understanding the mechanisms of self-assembly and thereby exerting full control over these processes. Traditional ensemble-based analysis methods often struggle to reveal critical features of the self-assembly that occur at the single particle level. Here, we describe a label-free single-particle assay to visualize real-time self-assembly in aqueous solutions by interferometric scattering microscopy. We demonstrate how the assay can be applied to biphasic reactions yielding micellar or vesicular aggregates, detecting the onset of aggregate formation, quantifying the kinetics at the single particle level, and distinguishing sigmoidal and exponential growth of aggregate populations. Furthermore, we can follow the evolution in aggregate size in real time, visualizing the nucleation stages of the self-assembly processes and record phenomena such as incorporation of oily components into the micelle or vesicle lumen.
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影响因子：
16.6
作者：
Morrow, Sarah M.;Colomer, Ignacio;Fletcher, Stephen P.
通讯作者：
Fletcher, Stephen P.
影响因子：
17.1
作者：
Loebling, Tina I.;Haataja, Johannes S.;Mueller, Axel H. E.
通讯作者：
Mueller, Axel H. E.
影响因子：
17.1
作者：
Biswal, Debasmita;Kusalik, Peter G.
通讯作者：
Kusalik, Peter G.
影响因子：
56.9
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Albertazzi, Lorenzo;van der Zwaag, Daan;Meijer, E. W.
通讯作者：
Meijer, E. W.
影响因子：
16.6
作者：
Bissette, Andrew J.;Odell, Barbara;Fletcher, Stephen P.
通讯作者：
Fletcher, Stephen P.