SUPPRESSOR OF PHYTOCHROME B-4 #3 reduces the expression of PIF-activated genes and increases expression of growth repressors to regulate hypocotyl elongation in short days.
SUPPRESSOR OF PHYTOCHROME B-4 #3 reduces the expression of PIF-activated genes and increases expression of growth repressors to regulate hypocotyl elongation in short days.
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植物色素B-4＃3的抑制剂降低了PIF激活基因的表达，并增加了生长抑制剂的表达，以在短几天内调节下胚轴伸长。
DOI：
10.1186/s12870-022-03737-z
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发表时间：
2022-08-15
影响因子：
5.3
通讯作者：
Neff, Michael M.
中科院分区：
文献类型：
--
作者：
Jacques, Caitlin N.;Favero, David S.;Kawamura, Ayako;Suzuki, Takamasa;Sugimoto, Keiko;Neff, Michael M.
植色素B-4的供应者#3（SOB 3）是包含核定位的AT-钩基序（阿勒）转录因子家族的成员，其参与拟南芥中光介导的生长，影响诸如下胚轴伸长的过程。大多数关于AHLs的研究都是在连续光照下进行的。然而，与恒定光照条件相比，存在独特的分子事件可以促进短日照（SD）内的生长。因此，我们研究了AHLs如何影响SD下胚轴伸长。首先，我们观察到AHLs抑制SD下胚轴的生长，类似于它们在恒定光照下的作用。接下来，我们通过在不同时间点在两个sob 3突变体中进行RNA-seq来鉴定SD生长的幼苗中的AHL调控基因。我们的转录组学数据表明，植物色素相互作用因子（PIF）4、5、7和8沿着PIF靶基因被SOB 3和/或其他AHL抑制。我们还鉴定了被抑制的PIF靶基因，这些基因以前没有被描述为AHL调节的，包括PRE 1、PIL 1、HFR 1、CDF 5和XTR 7。有趣的是，我们的RNA-seq数据还表明，AHLs激活生长阻遏物的表达以控制下胚轴伸长，如HY 5和IAA 17。值得注意的是，在测试的时间点，从RNA-seq实验中鉴定出的许多生长调节基因和其他基因在这两种sob 3突变体之间受到差异调节。令人惊讶的是，我们的ChIP-seq数据表明，SOB 3在一天中主要与相似的基因结合。总的来说，这些数据表明，AHLs影响基因表达的时间点特异性的方式，无论在整个SD结合DNA的变化。 在线版本包含补充材料，可通过10.1186/s12870-022-03737-z获得。
SUPPRESSOR OF PHYTOCHROME B-4 #3 (SOB3) is a member of the AT-HOOK MOTIF CONTAINING NUCLEAR LOCALIZED (AHL) family of transcription factors that are involved in light-mediated growth in Arabidopsis thaliana, affecting processes such as hypocotyl elongation. The majority of the research on the AHLs has been conducted in continuous light. However, there are unique molecular events that promote growth in short days (SD) compared to constant light conditions. Therefore, we investigated how AHLs affect hypocotyl elongation in SD. Firstly, we observed that AHLs inhibit hypocotyl growth in SD, similar to their effect in constant light. Next, we identified AHL-regulated genes in SD-grown seedlings by performing RNA-seq in two sob3 mutants at different time points. Our transcriptomic data indicate that PHYTOCHROME INTERACTING FACTORS (PIFs) 4, 5, 7, and 8 along with PIF-target genes are repressed by SOB3 and/or other AHLs. We also identified PIF target genes that are repressed and have not been previously described as AHL-regulated, including PRE1, PIL1, HFR1, CDF5, and XTR7. Interestingly, our RNA-seq data also suggest that AHLs activate the expression of growth repressors to control hypocotyl elongation, such as HY5 and IAA17. Notably, many growth-regulating and other genes identified from the RNA-seq experiment were differentially regulated between these two sob3 mutants at the time points tested. Surprisingly, our ChIP-seq data suggest that SOB3 mostly binds to similar genes throughout the day. Collectively, these data suggest that AHLs affect gene expression in a time point-specific manner irrespective of changes in binding to DNA throughout SD. The online version contains supplementary material available at 10.1186/s12870-022-03737-z.
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影响因子：
5.3
作者：
Bouallaga, I;Teissier, S;Thierry, F
通讯作者：
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