An Endothelial-to-Adipocyte Extracellular Vesicle Axis Governed by Metabolic State.
An Endothelial-to-Adipocyte Extracellular Vesicle Axis Governed by Metabolic State.
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DOI：
10.1016/j.cell.2018.09.005
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发表时间：
2018-10-18
期刊：
影响因子：
64.5
通讯作者：
Scherer PE
中科院分区：
文献类型：
--
作者：
Crewe C;Joffin N;Rutkowski JM;Kim M;Zhang F;Towler DA;Gordillo R;Scherer PE
我们发现了细胞外小泡(EV)在脂肪组织(AT)本身的细胞类型之间传递信号的存在。这一现象在我们试图产生脂肪细胞特异性的小窝蛋白1(CAV1)蛋白时变得明显起来。虽然我们有效地去除了脂肪细胞中的CAV1基因，但CAV1蛋白仍然丰富。通过使用新产生的小鼠模型，我们发现邻近的内皮细胞(ECs)在体内将含有CAV1的EV转移到脂肪细胞，脂肪细胞通过向ECs释放EV来相互作用。AT衍生的EV含有能够调节细胞信号通路的蛋白质和脂类。此外，这一机制还促进了血浆成分从内皮细胞向脂肪细胞的转移。这一转移事件受到禁食/再喂养和肥胖的生理调节，表明EVS参与了组织对全身营养状态变化的反应。这项工作为脂肪细胞、间质血管细胞和潜在的远端器官之间存在的复杂信号机制提供了新的见解。细胞外小泡在内皮细胞和脂肪细胞之间交换蛋白质和脂肪信号，传递脂肪组织中血液营养状态变化的信息
We have uncovered the existence of extracellular vesicle (EV)-mediated signaling between cell types within the adipose tissue (AT) proper. This phenomenon became evident in our attempts at generating an adipocyte-specific knock out of caveolin 1 (cav1) protein. While we effectively ablated the CAV1 gene in adipocytes, cav1 protein remained abundant. With the use of newly generated mouse models, we show that neighboring endothelial cells (ECs) transfer cav1-containing EVs to adipocytes in vivo, which reciprocate by releasing EVs to ECs. AT-derived EVs contain proteins and lipids capable of modulating cellular signaling pathways. Furthermore, this mechanism facilitates transfer of plasma constituents from ECs to the adipocyte. The transfer event is physiologically regulated by fasting/refeeding and obesity, suggesting EVs participate in the tissue response to changes in the systemic nutrient state. This work offers new insights into the complex signaling mechanisms that exist between adipocytes, stromal vascular cells and potentially distal organs. Extracellular vesicles exchange protein and lipid signals between endothelial cells and adipocytes conveying information about nutrient state changes from the blood in adipose tissues
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影响因子：
16
作者：
Lötvall J;Hill AF;Hochberg F;Buzás EI;Di Vizio D;Gardiner C;Gho YS;Kurochkin IV;Mathivanan S;Quesenberry P;Sahoo S;Tahara H;Wauben MH;Witwer KW;Théry C
通讯作者：
Théry C
DOI：
10.1073/pnas.1521230113
发表时间：
2016-02-23
影响因子：
11.1
作者：
Kowal, Joanna;Arras, Guillaume;Thery, Clotilde
通讯作者：
Thery, Clotilde
影响因子：
13.6
作者：
Conlan RS;Pisano S;Oliveira MI;Ferrari M;Mendes Pinto I
通讯作者：
Mendes Pinto I
影响因子：
29
作者：
Hla T;Dannenberg AJ
通讯作者：
Dannenberg AJ
使用可调的微流体系统，细胞外纳米植物和凋亡体的分离。
影响因子：
4.6
作者：
Shin S;Han D;Park MC;Mun JY;Choi J;Chun H;Kim S;Hong JW
通讯作者：
Hong JW