A deficiency screen of the 3rd chromosome for dominant modifiers of the Drosophila ER integral membrane protein, Jagunal.
A deficiency screen of the 3rd chromosome for dominant modifiers of the Drosophila ER integral membrane protein, Jagunal.
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DOI：
10.1093/g3journal/jkad059
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发表时间：
2023-07-05
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影响因子：
--
通讯作者：
中科院分区：
文献类型：
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细胞分裂过程中染色体遗传的机制已被很好地证明，然而，有丝分裂过程中的细胞器遗传却不太清楚。最近，内质网（ER）已被证明在有丝分裂期间重组，在细胞命运选择之前在前神经元细胞中不对称分裂，表明遗传的程序化机制。前神经细胞中的ER不对称分配依赖于高度保守的ER整合膜蛋白Jagunal（Jagn）。敲除果蝇复合眼中的Jagn，在48%的后代中显示多效性粗糙眼表型。为了鉴定参与Jagn依赖性ER分配途径的基因，我们对第3条染色体进行了显性修饰筛选，以寻找这种Jagn-RNAi诱导的粗糙眼表型的增强子和抑制子。我们筛选了181个覆盖3L和3R染色体的缺陷系，并鉴定了Jagn-RNAi表型的12个抑制子和10个增强子。基于缺陷所覆盖的基因的功能，我们鉴定了显示抑制或增强Jagn-RNAi表型的基因。这些包括分裂异常延迟（Dally），硫酸乙酰肝素蛋白聚糖，γ-分泌酶亚基早老素和ER驻留蛋白Sec 63。基于我们对这些靶点功能的理解，Jagn和Notch信号通路之间存在联系。进一步的研究将阐明Jagn的作用，并确定有丝分裂过程中ER分配机制中的相互作用。
The mechanism surrounding chromosome inheritance during cell division has been well documented, however, organelle inheritance during mitosis is less understood. Recently, the endoplasmic reticulum (ER) has been shown to reorganize during mitosis, dividing asymmetrically in proneuronal cells prior to cell fate selection, indicating a programmed mechanism of inheritance. ER asymmetric partitioning in proneural cells relies on the highly conserved ER integral membrane protein, Jagunal (Jagn). Knockdown of Jagn in the compound Drosophila eye displays a pleotropic rough eye phenotype in 48% of the progeny. To identify genes involved in Jagn dependent ER partitioning pathway, we performed a dominant modifier screen of the 3rd chromosome for enhancers and suppressors of this Jagn-RNAi-induced rough eye phenotype. We screened through 181 deficiency lines covering the 3L and 3R chromosomes and identified 12 suppressors and 10 enhancers of the Jagn-RNAi phenotype. Based on the functions of the genes covered by the deficiencies, we identified genes that displayed a suppression or enhancement of the Jagn-RNAi phenotype. These include Division Abnormally Delayed (Dally), a heparan sulfate proteoglycan, the γ-secretase subunit Presenilin, and the ER resident protein Sec63. Based on our understanding of the function of these targets, there is a connection between Jagn and the Notch signaling pathway. Further studies will elucidate the role of Jagn and identified interactors within the mechanisms of ER partitioning during mitosis.
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影响因子：
30.8
作者：
Boztug K;Järvinen PM;Salzer E;Racek T;Mönch S;Garncarz W;Gertz EM;Schäffer AA;Antonopoulos A;Haslam SM;Schieck L;Puchałka J;Diestelhorst J;Appaswamy G;Lescoeur B;Giambruno R;Bigenzahn JW;Elling U;Pfeifer D;Conde CD;Albert MH;Welte K;Brandes G;Sherkat R;van der Werff Ten Bosch J;Rezaei N;Etzioni A;Bellanné-Chantelot C;Superti-Furga G;Penninger JM;Bennett KL;von Blume J;Dell A;Donadieu J;Klein C
通讯作者：
Klein C
在果蝇卵子发生过程中，jagunal是重组内质网所必需的。
影响因子：
7.8
作者：
Lee, Sangil;Cooley, Lynn
通讯作者：
Cooley, Lynn
影响因子：
2.5
作者：
Kumar, Justin P.
通讯作者：
Kumar, Justin P.
影响因子：
3.3
作者：
Gramates LS;Agapite J;Attrill H;Calvi BR;Crosby MA;Dos Santos G;Goodman JL;Goutte-Gattat D;Jenkins VK;Kaufman T;Larkin A;Matthews BB;Millburn G;Strelets VB;the FlyBase Consortium
通讯作者：
the FlyBase Consortium
影响因子：
3.3
作者：
Eritano AS;Altamirano A;Beyeler S;Gaytan N;Velasquez M;Riggs B
通讯作者：
Riggs B