Rab11 mediates selective recycling and endocytic trafficking in Trypanosoma brucei.
Rab11 mediates selective recycling and endocytic trafficking in Trypanosoma brucei.
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DOI：
10.1111/tra.12565
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发表时间：
2018-06
期刊：
影响因子：
--
通讯作者：
Bangs JD
中科院分区：
文献类型：
--
作者：
Umaer K;Bush PJ;Bangs JD
Trypanosoma brucei possesses a streamlined secretory system that guarantees efficient delivery to the cell surface of the critical GPI-anchored virulence factors, variable surface glycoprotein (VSG) and transferrin receptor (TfR). Both are thought to be constitutively endocytosed and returned to the flagellar pocket via TbRab11+ recycling endosomes. We use conditional knockdown with established reporters to investigate the role of TbRab11 in specific endomembrane trafficking pathways in bloodstream trypanosomes. TbRab11 is essential. Ablation has a modest negative effect on general endocytosis, but does not affect turnover, steady state levels, or surface localization of TfR. Nor are biosynthetic delivery to the cell surface and recycling of VSG affected. TbRab11 depletion also causes increased shedding of VSG into the media by formation of extracellular vesicles. In contrast to GPI-anchored cargo, TbRab11 depletion reduces recycling of the trans-membrane invariant surface protein, ISG65, leading to increased lysosomal turnover. Thus, TbRab11 plays a critical role in recycling of transmembrane, but not GPI-anchored surface proteins. We proposed a two-step model for VSG turnover involving release of VSG containing vesicles followed by GPI-hydrolysis. Collectively, our results indicate a critical role of TbRab11 in the homeostatic maintenance of the secretory/endocytic system of bloodstream T. brucei.
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DOI：
10.1111/tra.12016
发表时间：
2013-01
期刊：
Traffic (Copenhagen, Denmark)
影响因子：
--
作者：
Beckett K;Monier S;Palmer L;Alexandre C;Green H;Bonneil E;Raposo G;Thibault P;Le Borgne R;Vincent JP
通讯作者：
Vincent JP
影响因子：
64.5
作者：
Engstler, Markus;Pfohl, Thomas;Overath, Peter
通讯作者：
Overath, Peter
影响因子：
1.5
作者：
Ackers, JP;Dhir, V;Field, MC
通讯作者：
Field, MC
影响因子：
1.5
作者：
Burkard, Gabriela;Fragoso, Cristina M.;Roditi, Isabel
通讯作者：
Roditi, Isabel
影响因子：
1.5
作者：
Gilden, Julia K.;Umaer, Khan;Bangs, James D.
通讯作者：
Bangs, James D.