The ϕPA3 phage nucleus is enclosed by a self-assembling 2D crystalline lattice.
The ϕPA3 phage nucleus is enclosed by a self-assembling 2D crystalline lattice.
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DOI：
10.1038/s41467-023-36526-9
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发表时间：
2023-02-18
影响因子：
16.6
通讯作者：
Agard, David A.
中科院分区：
文献类型：
--
作者：
Nieweglowska, Eliza S.;Brilot, Axel F.;Mendez-Moran, Melissa;Kokontis, Claire;Baek, Minkyung;Li, Junrui;Cheng, Yifan;Baker, David;Bondy-Denomy, Joseph;Agard, David A.
为了保护自己免受宿主的攻击，许多巨型噬菌体建立了一个噬菌体核--一个微米级的蛋白质结构，包围着复制的噬菌体DNA。与复制和转录相关的噬菌体和宿主蛋白集中在噬菌体细胞核内，而其他噬菌体和宿主蛋白则被排除在外，包括CRISPR-Cas和限制性内切酶宿主防御系统。在这里，我们发现从感染ϕPA3的铜绿假单胞菌中分离出的细胞核片段形成了具有p2或p4对称性的二维晶格。我们进一步证明了重组纯化的初级噬菌体核壳蛋白(PHUN)自发组装成具有p2和p4对称性的类似2D片断。我们用低温电子显微镜将占主导地位的p2对称态分解为3.9 ä。我们的结构揭示了一个由两个磁区组成的准对称四聚体结构。柔性环和末端调节可调节的四聚体间接触，从而驱动亚单位组装成晶格并能够采用不同的对称状态。虽然亚基之间的界面大多是紧密排列的，但有两个是开放的，形成的通道可能对蛋白质、信使核糖核酸和小分子的运输具有功能意义。为了防止宿主攻击，许多巨型噬菌体建立了一个微米级的基于蛋白质的结构来封闭它们复制的DNA。利用低温电子显微镜，作者证明包裹着这个所谓的噬菌体细胞核的2D晶体是由柔性环和尾巴连接的四聚体组装而成。
To protect themselves from host attack, numerous jumbo bacteriophages establish a phage nucleus—a micron-scale, proteinaceous structure encompassing the replicating phage DNA. Bacteriophage and host proteins associated with replication and transcription are concentrated inside the phage nucleus while other phage and host proteins are excluded, including CRISPR-Cas and restriction endonuclease host defense systems. Here, we show that nucleus fragments isolated from ϕPA3 infected Pseudomonas aeruginosa form a 2-dimensional lattice, having p2 or p4 symmetry. We further demonstrate that recombinantly purified primary Phage Nuclear Enclosure (PhuN) protein spontaneously assembles into similar 2D sheets with p2 and p4 symmetry. We resolve the dominant p2 symmetric state to 3.9 Å by cryo-EM. Our structure reveals a two-domain core, organized into quasi-symmetric tetramers. Flexible loops and termini mediate adaptable inter-tetramer contacts that drive subunit assembly into a lattice and enable the adoption of different symmetric states. While the interfaces between subunits are mostly well packed, two are open, forming channels that likely have functional implications for the transport of proteins, mRNA, and small molecules. To protect from host attack, numerous jumbo bacteriophages establish a micron-scale, protein-based structure to enclose their replicating DNA. Using cryoEM, the authors show that the 2D crystal enclosing this so-called phage nucleus is an assembly of tetramers linked by flexible loops and tails.
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影响因子：
5.7
作者：
Arce-Rodriguez, Alejandro;Volke, Daniel C.;Nikel, Pablo I.
通讯作者：
Nikel, Pablo I.
DOI：
10.1107/s2059798318009324
发表时间：
2018-09-01
期刊：
Acta crystallographica. Section D, Structural biology
影响因子：
--
作者：
Afonine PV;Klaholz BP;Moriarty NW;Poon BK;Sobolev OV;Terwilliger TC;Adams PD;Urzhumtsev A
通讯作者：
Urzhumtsev A
影响因子：
64.8
作者：
Jumper J;Evans R;Pritzel A;Green T;Figurnov M;Ronneberger O;Tunyasuvunakool K;Bates R;Žídek A;Potapenko A;Bridgland A;Meyer C;Kohl SAA;Ballard AJ;Cowie A;Romera-Paredes B;Nikolov S;Jain R;Adler J;Back T;Petersen S;Reiman D;Clancy E;Zielinski M;Steinegger M;Pacholska M;Berghammer T;Bodenstein S;Silver D;Vinyals O;Senior AW;Kavukcuoglu K;Kohli P;Hassabis D
通讯作者：
Hassabis D
DOI：
10.1073/pnas.2108738118
发表时间：
2021-12-14
影响因子：
11.1
作者：
Croxford M;Elbaum M;Arigovindan M;Kam Z;Agard D;Villa E;Sedat J
通讯作者：
Sedat J
影响因子：
64.8
作者：
Mendoza, Senen D.;Nieweglowska, Eliza S.;Bondy-Denomy, Joseph
通讯作者：
Bondy-Denomy, Joseph