Relationship between oxidative stress and lifespan in Daphnia pulex.
Relationship between oxidative stress and lifespan in Daphnia pulex.
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DOI：
10.1038/s41598-022-06279-4
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发表时间：
2022-02-11
影响因子：
4.6
通讯作者：
Patel RC
中科院分区：
文献类型：
--
作者：
Ukhueduan B;Schumpert C;Kim E;Dudycha JL;Patel RC
导致细胞、组织和最终器官功能障碍的大分子损伤是衰老的主要原因。正常代谢产生的细胞内活性氧（ROS）对大分子物质的损伤最大，而线粒体是ROS的主要来源，在这一过程中起着核心作用。线粒体（MT）基因组中自然发生的变异导致相应的更少或更多的ROS和大分子损伤，改变衰老相关的生物体衰退的速度之间的关系仍然相对未被探索。MT复合物I是电子传递链（ETC）的组分，是ROS的关键来源，并且NADH脱氢酶亚基5（ND5）是构成复合物I骨架的亚基的高度保守的核心蛋白。使用水蚤作为模式生物，我们探索了ND5中自然发生的序列变异是否与短或长的寿命相关。我们的研究结果表明，短寿命的克隆ND5的变体与降低复合物I的活性，增加氧化损伤，提高表达的ROS清除酶。水蚤提供了一个独特的机会，调查复杂的I和ROS产生的影响衰老和寿命的速度的组件之间的关联遗传变异。
Macromolecular damage leading to cell, tissue and ultimately organ dysfunction is a major contributor to aging. Intracellular reactive oxygen species (ROS) resulting from normal metabolism cause most damage to macromolecules and the mitochondria play a central role in this process as they are the principle source of ROS. The relationship between naturally occurring variations in the mitochondrial (MT) genomes leading to correspondingly less or more ROS and macromolecular damage that changes the rate of aging associated organismal decline remains relatively unexplored. MT complex I, a component of the electron transport chain (ETC), is a key source of ROS and the NADH dehydrogenase subunit 5 (ND5) is a highly conserved core protein of the subunits that constitute the backbone of complex I. Using Daphnia as a model organism, we explored if the naturally occurring sequence variations in ND5 correlate with a short or long lifespan. Our results indicate that the short-lived clones have ND5 variants that correlate with reduced complex I activity, increased oxidative damage, and heightened expression of ROS scavenger enzymes. Daphnia offers a unique opportunity to investigate the association between inherited variations in components of complex I and ROS generation which affects the rate of aging and lifespan.
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