Transcriptional response of Streptococcus pneumoniae to Zn2+) limitation and the repressor/activator function of AdcR.
Transcriptional response of Streptococcus pneumoniae to Zn2+) limitation and the repressor/activator function of AdcR.
复制标题
肺炎链球菌对 Zn2+ 限制的转录反应和 AdcR 的阻遏/激活功能。
作者：
Sulman Shafeeq;Tomas G. Kloosterman;O. Kuipers
锌 (Zn(2+)) 是一种重要的微量金属离子，已被证明可以调节链球菌中多种（毒力）基因的表达。之前，我们分析了肺炎链球菌对 Zn(2+) 胁迫的全基因组反应。在这项工作中，我们进行了转录组分析，以确定在细胞内 Zn(2+) 限制下差异表达的基因。这揭示了许多在缺乏细胞外Zn（2+）的情况下高度上调的基因，其中属于Zn（2+）响应阻遏物AdcR调节子的基因，如编码Zn（2+）依赖性ABC摄取系统的adcBCA，编码Zn（2+）结合脂蛋白的adcAII，以及属于Pht家族（phtA， phtB、phtD 和 phtE）。通过转录组分析、lacZ 报告基因研究、体外 DNA 结合实验和计算机模拟预测，我们发现在 Zn(2+) 存在的情况下，AdcR 直接抑制 adcRCBA、adcAII-phtD、phtA、phtB 和 phtE 的启动子。 AdcR 还可以充当激活剂，因为在 Zn(2+) 存在的情况下，它直接诱导编码含 Zn(2+) 的乙醇脱氢酶的 adh 表达。总之，建立了肺炎链球菌对Zn(2+)限制的全基因组转录反应，该反应主要通过Zn(2+)依赖性调节因子AdcR的直接调节介导。
Zinc (Zn(2+)) is an important trace metal ion that has been shown to regulate the expression of several (virulence) genes in streptococci. Previously, we analyzed the genome-wide response of S. pneumoniae to Zn(2+)-stress. In this work, we have performed a transcriptomic analysis to identify genes that are differentially expressed under intracellular Zn(2+) limitation. This revealed a number of genes that are highly upregulated in the absence of extracellular Zn(2+), amongst which the genes belonging to the regulon of the Zn(2+)-responsive repressor AdcR, like adcBCA, encoding a Zn(2+)-dependent ABC-uptake system, adcAII, encoding a Zn(2+)-binding lipoprotein, and also virulence genes belonging to the Pht family (phtA, phtB, phtD and phtE). Using transcriptome analysis, lacZ-reporter studies, in vitro DNA binding experiments, and in silico operator predictions, we show that AdcR directly represses the promoters of adcRCBA, adcAII-phtD, phtA, phtB and phtE in the presence of Zn(2+). AdcR can also function as an activator, since in the presence of Zn(2+) it directly induces expression of adh that encodes a Zn(2+)-containing alcohol dehydrogenase. In conclusion, the genome-wide transcriptional response of S. pneumoniae to Zn(2+) limitation was established, which is mainly mediated via direct regulation by the Zn(2+)-dependent regulator AdcR.
戈登链球菌生物膜形成中 adcCBA 基因的突变分析。
DOI：
10.1111/j.1399-302x.2004.00205.x
发表时间：
2005
期刊：
Oral microbiology and immunology.
影响因子：
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作者：
Mitrakul,K;Loo,CY;Gyurko,C;Hughes,CV;Ganeshkumar,N
通讯作者：
Ganeshkumar,N