Restraint stress activates nesfatin-1-immunoreactive brain nuclei in rats.
Restraint stress activates nesfatin-1-immunoreactive brain nuclei in rats.
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DOI：
10.1016/j.brainres.2009.08.082
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发表时间：
2009-12-01
期刊：
影响因子：
2.9
通讯作者：
Taché Y
中科院分区：
文献类型：
--
作者：
Goebel M;Stengel A;Wang L;Taché Y
Nesfatin-1是一种新发现的肽，据报道，当中央注射时，可以减少食物摄入。我们最近描述了它在大鼠脑自主神经核中的广泛分布，这意味着应激可能会招募nesfatin-1。我们研究了是否约束，一个混合的心理和生理应激激活nesfatin-1免疫反应（ir）神经元在大鼠大脑。雄性Sprague-Dawley大鼠进行30分钟的约束或不受干扰，90分钟后，大脑进行处理，用于Fos和nesfatin-1的双重免疫组织化学标记。束缚诱导Fos在视上核（SON）、室旁核（PVN）、蓝斑（LC）、苍白中缝吻端（rRPa）、孤束核（NTS）和延髓腹外侧区（VLM）神经元表达。Fos/nesfatin-1双标记结果显示，SON、VLM、LC、NTS、rRPa、小细胞室旁核、内侧大细胞室旁核、外侧大细胞室旁核和内侧小细胞室旁核中的Fos阳性神经元分别占nesfatin-1阳性神经元的95%、90%、80%、48%、57%、48%和27%。这些数据表明，nesfatin-1神经元是下丘脑和后脑神经元细胞群的一部分，通过限制激活，这表明nesfatin-1在应激反应中的可能作用。
Nesfatin-1 is a newly discovered peptide that was reported to reduce food intake when injected centrally. We recently described its wide distribution in rat brain autonomic nuclei which implies potential recruitment of nesfatin-1 by stress. We investigated whether restraint, a mixed psychological and physical stressor activates nesfatin-1-immunoreactive (ir) neurons in the rat brain. Male Sprague-Dawley rats were either subjected to 30 min restraint or left undisturbed and 90 min later brains were processed for double immunohistochemical labeling of Fos and nesfatin-1. Restraint induced significant Fos expression in neurons of the supraoptic nucleus (SON), paraventricular nucleus (PVN), locus coeruleus (LC), rostral raphe pallidus (rRPa), nucleus of the solitary tract (NTS) and ventrolateral medulla (VLM). Double Fos/nesfatin-1 labeling revealed that Fos-ir neurons comprised 95% of nesfatin-1-ir cells in the SON, 90% in the VLM, 80% in the LC, 48% in the caudal NTS, 57% in the rRPa, 48% in the anterior parvicellular PVN, 27% in the medial magnocellular PVN, 18% in the lateral magnocellular PVN and 10% in the medial parvicellular PVN. These data demonstrate that nesfatin-1 neurons are part of the hypothalamic and hindbrain neuronal cell groups activated by restraint suggesting a possible role of nesfatin-1 in the response to stress.
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