Rubisco Adaptation Is More Limited by Phylogenetic Constraint Than by Catalytic Trade-off.
Rubisco Adaptation Is More Limited by Phylogenetic Constraint Than by Catalytic Trade-off.
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DOI：
10.1093/molbev/msab079
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发表时间：
2021-06-25
影响因子：
10.7
通讯作者：
Kelly S
中科院分区：
文献类型：
--
作者：
Bouvier JW;Emms DM;Rhodes T;Bolton JS;Brasnett A;Eddershaw A;Nielsen JR;Unitt A;Whitney SM;Kelly S
Rubisco吸收CO2形成糖，为地球上的生命提供燃料。跨物种的rubisco动力学性状之间的相关性导致的命题，rubisco的适应是高度限制的催化权衡。然而，这些分析没有考虑所分析的酶的系统发育背景。因此，这是可能的，观察到的相关性是一个人工制品的存在系统发育信号rubisco动力学和采样的物种之间的系统发育关系。在这里，我们进行了rubisco动力学的遗传学解析分析，并表明在rubisco动力学性状中存在显着的系统发育信号。我们重新评估了这种系统发育信号的催化权衡的程度，发现所有这些都被减弱了。系统发育校正后，最大的催化权衡之间观察到的米氏常数为CO2和羧化酶营业额（1021 -37%），和米氏常数为CO2和O2（1099 -19%），分别。所有其他催化权衡基本上减弱，使得它们是边际的（<9%）或不显著的。这种rubisco动力学演化的遗传学解析分析还确定了伴随C4光合作用演化而发生的动力学变化。最后，我们表明，系统发育的限制发挥了更大的作用比催化权衡限制rubisco动力学的演变。因此，虽然有强有力的证据表明，一些催化权衡，rubisco适应已受到系统发育的限制比所有催化权衡的联合行动。
Rubisco assimilates CO2 to form the sugars that fuel life on earth. Correlations between rubisco kinetic traits across species have led to the proposition that rubisco adaptation is highly constrained by catalytic trade-offs. However, these analyses did not consider the phylogenetic context of the enzymes that were analyzed. Thus, it is possible that the correlations observed were an artefact of the presence of phylogenetic signal in rubisco kinetics and the phylogenetic relationship between the species that were sampled. Here, we conducted a phylogenetically resolved analysis of rubisco kinetics and show that there is a significant phylogenetic signal in rubisco kinetic traits. We re-evaluated the extent of catalytic trade-offs accounting for this phylogenetic signal and found that all were attenuated. Following phylogenetic correction, the largest catalytic trade-offs were observed between the Michaelis constant for CO2 and carboxylase turnover (∼21–37%), and between the Michaelis constants for CO2 and O2 (∼9–19%), respectively. All other catalytic trade-offs were substantially attenuated such that they were marginal (<9%) or non-significant. This phylogenetically resolved analysis of rubisco kinetic evolution also identified kinetic changes that occur concomitant with the evolution of C4 photosynthesis. Finally, we show that phylogenetic constraints have played a larger role than catalytic trade-offs in limiting the evolution of rubisco kinetics. Thus, although there is strong evidence for some catalytic trade-offs, rubisco adaptation has been more limited by phylogenetic constraint than by the combined action of all catalytic trade-offs.
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影响因子：
3.7
作者：
Carlin DA;Caster RW;Wang X;Betzenderfer SA;Chen CX;Duong VM;Ryklansky CV;Alpekin A;Beaumont N;Kapoor H;Kim N;Mohabbot H;Pang B;Teel R;Whithaus L;Tagkopoulos I;Siegel JB
通讯作者：
Siegel JB
影响因子：
56.9
作者：
Beer, Christian;Reichstein, Markus;Papale, Dario
通讯作者：
Papale, Dario
影响因子：
3.3
作者：
CHEVERUD, JM;DOW, MM;LEUTENEGGER, W
通讯作者：
LEUTENEGGER, W
DOI：
10.1007/bf00272157
发表时间：
1991-10-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
CLETONJANSEN, AM;DEKKER, S;GOOSEN, N
通讯作者：
GOOSEN, N
影响因子：
3.9
作者：
BADGER, MR;LORIMER, GH
通讯作者：
LORIMER, GH