Defining early steps in Bacillus subtilis biofilm biosynthesis.
Defining early steps in Bacillus subtilis biofilm biosynthesis.
复制标题
DOI：
10.1128/mbio.00948-23
复制
发表时间：
2023-10-31
期刊：
影响因子：
6.4
通讯作者：
Imperiali, Barbara
中科院分区：
文献类型：
--
作者：
Arbour, Christine A.;Nagar, Rupa;Bernstein, Hannah M.;Ghosh, Soumi;Al-Sammarraie, Yusra;Dorfmueller, Helge C.;Ferguson, Michael A. J.;Stanley-Wall, Nicola R.;Imperiali, Barbara
枯草芽孢杆菌细胞外生物膜基质包括胞外多糖（EPS），其对于群落的结构和功能至关重要。迄今为止，我们对B的生物合成机制和EPS分子组成的了解。subtilis仍然不清楚和不完整。本报告提出了协同生物化学和遗传研究建立在基础上的比较序列分析，旨在阐明活动的前两个膜承诺的步骤，在EPS生物合成途径。通过采用这种方法，我们确定了B中前两种酶的核苷酸糖供体和脂质连接的受体底物。枯草杆菌生物膜EPS生物合成途径。EpsL使用尿苷二磷酸（UDP）-二-N-乙酰基杆菌胺作为磷酸糖供体催化第一个磷酸糖基转移酶步骤。EpsD是预测的保留糖基转移酶的GT-B折叠（GT 4家族），其催化利用EpsL的产物作为受体底物和UDP-N-乙酰葡糖胺作为糖供体的途径中的第二步。因此，该研究定义了不断增长的EPS单元还原端的前两个单糖。在这样做的过程中，我们提供了第一个证据的存在下，杆菌胺的EPS合成的革兰氏阳性菌。生物膜是微生物为增加生存而采取的共同生活方式。我们能够系统地促进或消融生物膜形成的关键是对生物膜基质大分子的详细了解。在这里，我们确定了枯草芽孢杆菌生物膜基质胞外多糖（EPS）合成途径中的前两个重要步骤。总之，我们的研究和方法为EPS生物合成步骤的顺序表征提供了基础，使用先前的步骤来实现十一异戊二烯二磷酸连接的聚糖底物的化学酶促合成。
The Bacillus subtilis extracellular biofilm matrix includes an exopolysaccharide (EPS) that is critical for the architecture and function of the community. To date, our understanding of the biosynthetic machinery and the molecular composition of the EPS of B. subtilis remains unclear and incomplete. This report presents synergistic biochemical and genetic studies built from a foundation of comparative sequence analyses targeted at elucidating the activities of the first two membrane-committed steps in the EPS biosynthetic pathway. By taking this approach, we determined the nucleotide sugar donor and lipid-linked acceptor substrates for the first two enzymes in the B. subtilis biofilm EPS biosynthetic pathway. EpsL catalyzes the first phosphoglycosyl transferase step using uridine diphosphate (UDP)-di-N-acetyl bacillosamine as phospho-sugar donor. EpsD is a predicted GT-B fold (GT4 family) retaining glycosyl transferase that catalyzes the second step in the pathway that utilizes the product of EpsL as an acceptor substrate and UDP-N-acetyl glucosamine as the sugar donor. Thus, the study defines the first two monosaccharides at the reducing end of the growing EPS unit. In doing so, we provide the first evidence of the presence of bacillosamine in an EPS synthesized by a Gram-positive bacterium. Biofilms are the communal way of life that microbes adopt to increase survival. Key to our ability to systematically promote or ablate biofilm formation is a detailed understanding of the biofilm matrix macromolecules. Here, we identify the first two essential steps in the Bacillus subtilis biofilm matrix exopolysaccharide (EPS) synthesis pathway. Together, our studies and approaches provide the foundation for the sequential characterization of the steps in EPS biosynthesis, using prior steps to enable chemoenzymatic synthesis of the undecaprenyl diphosphate-linked glycan substrates.
登录
查看更多内容
影响因子：
11
作者：
Eigentler, Lukas;Kalamara, Margarita;Ball, Graeme;MacPhee, Cait E.;Stanley-Wall, Nicola R.;Davidson, Fordyce A.
通讯作者：
Davidson, Fordyce A.
影响因子：
6.4
作者：
Chai Y;Beauregard PB;Vlamakis H;Losick R;Kolter R
通讯作者：
Kolter R
影响因子：
64.8
作者：
Jumper J;Evans R;Pritzel A;Green T;Figurnov M;Ronneberger O;Tunyasuvunakool K;Bates R;Žídek A;Potapenko A;Bridgland A;Meyer C;Kohl SAA;Ballard AJ;Cowie A;Romera-Paredes B;Nikolov S;Jain R;Adler J;Back T;Petersen S;Reiman D;Clancy E;Zielinski M;Steinegger M;Pacholska M;Berghammer T;Bodenstein S;Silver D;Vinyals O;Senior AW;Kavukcuoglu K;Kohli P;Hassabis D
通讯作者：
Hassabis D
影响因子：
8
作者：
Anderson, Alyssa J. J.;Dodge, Greg J. J.;Allen, Karen N. N.;Imperiali, Barbara
通讯作者：
Imperiali, Barbara
DOI：
10.1073/pnas.1103321108
发表时间：
2011-06-07
影响因子：
11.1
作者：
Borud, Bente;Viburiene, Raimonda;Koomey, Michael
通讯作者：
Koomey, Michael