Founder cell configuration drives competitive outcome within colony biofilms.
Founder cell configuration drives competitive outcome within colony biofilms.
复制标题
DOI：
10.1038/s41396-022-01198-8
复制
发表时间：
2022-06
期刊：
影响因子：
11
通讯作者：
Davidson, Fordyce A.
中科院分区：
文献类型：
--
作者：
Eigentler, Lukas;Kalamara, Margarita;Ball, Graeme;MacPhee, Cait E.;Stanley-Wall, Nicola R.;Davidson, Fordyce A.
细菌可以形成密集的群落，称为生物膜，其中细胞嵌入在自我产生的细胞外基质中。利用生物膜环境中菌株之间的竞争性相互作用在生物、医学和工业系统中具有潜在的应用。通过将数学模型与实验分析相结合，我们发现用于接种生物膜的创始细胞的位置和密度显著影响生物膜内的空间结构和竞争动态。利用描述菌落生物膜形成的物种独立理论框架，我们发现在由两个等基因菌株组成的成熟生物膜中观察到的空间结构和相对菌株生物量可以直接映射到奠基细胞的地理分布。此外，我们定义了一个竞争结果的预测器，该预测器仅基于创始细胞的径向扩张潜力准确地预测菌株的相对丰度。因此，我们揭示了在低奠基者密度下接种的生物膜中竞争结果的可变性是奠基者细胞在接种物中随机定位的自然结果。将我们的研究扩展到通过局部拮抗相互作用的非等基因菌株，表明即使对于具有不同竞争优势的菌株，在低founder密度生物膜中，争夺空间仍然是主要的竞争模式。我们的研究结果通过枯草芽孢杆菌的实验分析得到验证，强调了空间动力学对生物膜内竞争相互作用的重要性，因此对相关应用具有重要意义。
Bacteria can form dense communities called biofilms, where cells are embedded in a self-produced extracellular matrix. Exploiting competitive interactions between strains within the biofilm context can have potential applications in biological, medical, and industrial systems. By combining mathematical modelling with experimental assays, we reveal that spatial structure and competitive dynamics within biofilms are significantly affected by the location and density of the founder cells used to inoculate the biofilm. Using a species-independent theoretical framework describing colony biofilm formation, we show that the observed spatial structure and relative strain biomass in a mature biofilm comprising two isogenic strains can be mapped directly to the geographical distributions of founder cells. Moreover, we define a predictor of competitive outcome that accurately forecasts relative abundance of strains based solely on the founder cells’ potential for radial expansion. Consequently, we reveal that variability of competitive outcome in biofilms inoculated at low founder density is a natural consequence of the random positioning of founding cells in the inoculum. Extension of our study to non-isogenic strains that interact through local antagonisms, shows that even for strains with different competition strengths, a race for space remains the dominant mode of competition in low founder density biofilms. Our results, verified by experimental assays using Bacillus subtilis, highlight the importance of spatial dynamics on competitive interactions within biofilms and hence to related applications.
登录
查看更多内容
DOI：
10.1073/pnas.1212429109
发表时间：
2012-11-13
影响因子：
11.1
作者：
Asally, Munehiro;Kittisopikul, Mark;Sueel, Guerol M.
通讯作者：
Sueel, Guerol M.
影响因子：
64.8
作者：
Battin, TJ;Kaplan, LA;Hansen, CME
通讯作者：
Hansen, CME
影响因子：
16.8
作者：
Dal Co, Alma;van Vliet, Simon;Ackermann, Martin
通讯作者：
Ackermann, Martin
DOI：
10.1073/pnas.0710150104
发表时间：
2007-12-11
影响因子：
11.1
作者：
Hallatschek, Oskar;Hersen, Pascal;Nelson, David R.
通讯作者：
Nelson, David R.
影响因子：
28.3
作者：
Hartmann R;Jeckel H;Jelli E;Singh PK;Vaidya S;Bayer M;Rode DKH;Vidakovic L;Díaz-Pascual F;Fong JCN;Dragoš A;Lamprecht O;Thöming JG;Netter N;Häussler S;Nadell CD;Sourjik V;Kovács ÁT;Yildiz FH;Drescher K
通讯作者：
Drescher K