Plasma MicroRNA Profiling of Plasmodium falciparum Biomass and Association with Severity of Malaria Disease.
Plasma MicroRNA Profiling of Plasmodium falciparum Biomass and Association with Severity of Malaria Disease.
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DOI：
10.3201/eid2702.191795
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发表时间：
2021-03
影响因子：
11.8
通讯作者：
Mayor A
中科院分区：
文献类型：
--
作者：
Gupta H;Rubio M;Sitoe A;Varo R;Cisteró P;Madrid L;Cuamba I;Jimenez A;Martiáñez-Vendrell X;Barrios D;Pantano L;Brimacombe A;Bustamante M;Bassat Q;Mayor A
严重疟疾（SM）是疟疾流行国家的一个主要公共卫生问题。恶性疟原虫感染的红细胞在重要器官中的隔离和相关的炎症导致器官功能障碍。微小RNA（microRNAs，miRNAs）从受损组织中迅速释放到宿主体液中，是SM预后的一个有前景的生物标志物。我们应用新一代测序技术来评估SM和单纯性疟疾（UM）中miRNA的差异表达。六种miRNAs与体外恶性疟原虫细胞粘附、儿童严重程度和恶性疟原虫生物量相关。通过TaqMan-定量逆转录PCR定量的hsa-miR-4497的相对表达在SM儿童的血浆中高于UM儿童（p<0.048），并且再次与恶性疟原虫生物量相关（p = 0.033）。这些发现表明SM和UM中不同的生理病理过程导致miRNA的差异表达，并为未来研究评估其在疟疾中的预后价值铺平了道路。
Severe malaria (SM) is a major public health problem in malaria-endemic countries. Sequestration of Plasmodium falciparum–infected erythrocytes in vital organs and the associated inflammation leads to organ dysfunction. MicroRNAs (miRNAs), which are rapidly released from damaged tissues into the host fluids, constitute a promising biomarker for the prognosis of SM. We applied next-generation sequencing to evaluate the differential expression of miRNAs in SM and in uncomplicated malaria (UM. Six miRNAs were associated with in vitro P. falciparum cytoadhesion, severity in children, and P. falciparum biomass. Relative expression of hsa-miR-4497 quantified by TaqMan-quantitative reverse transcription PCR was higher in plasma of children with SM than those with UM (p<0.048) and again correlated with P. falciparum biomass (p = 0.033). These findings suggest that different physiopathological processes in SM and UM lead to differential expression of miRNAs and pave the way for future studies to assess their prognostic value in malaria.
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影响因子：
3.7
作者：
Mayor A;Hafiz A;Bassat Q;Rovira-Vallbona E;Sanz S;Machevo S;Aguilar R;Cisteró P;Sigaúque B;Menéndez C;Alonso PL;Chitnis CE
通讯作者：
Chitnis CE
影响因子：
3.7
作者：
Kirschner MB;Edelman JJ;Kao SC;Vallely MP;van Zandwijk N;Reid G
通讯作者：
Reid G
影响因子：
9.5
作者：
Marabita F;de Candia P;Torri A;Tegnér J;Abrignani S;Rossi RL
通讯作者：
Rossi RL
DOI：
10.1073/pnas.95.13.7620
发表时间：
1998-06-23
影响因子：
11.1
作者：
Gravenor, MB;van Hensbroek, MB;Kwiatkowski, D
通讯作者：
Kwiatkowski, D
影响因子：
3.8
作者：
Baro, Barbara;Deroost, Katrien;del Portillo, Hernando A.
通讯作者：
del Portillo, Hernando A.