Neutralizing activity of the antibodies against two kinds of envelope glycoproteins of Sendai virus
Neutralizing activity of the antibodies against two kinds of envelope glycoproteins of Sendai virus
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仙台病毒两种包膜糖蛋白抗体的中和活性
DOI：
10.1007/bf01316735
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发表时间：
2005
影响因子：
2.7
通讯作者：
M. Arifuku
中科院分区：
文献类型：
--
作者：
H. Tozawa;H. Komatsu;K. Ohkata;T. Nakajima;M. Watanabe;Y. Tanaka;M. Arifuku
摘要制备了仙台病毒（SV）融合蛋白(F)和血凝素神经氨酸酶（HN）的小鼠单克隆抗体，并研究了它们的抗病毒活性，特别是对感染性的中和作用。固相竞争ELISA法将26种抗hn抗体至少分为4组（HN-I、-II、-III和-IV）。hn - 1， -II和-III组抗体识别的抗原位点在地形上彼此分离。HN-IV组抗体识别的位点与HN-I、HN-II和hn -III组抗体识别的位点部分重叠。属于HN-III组的抗体高度中和SV的传染性，弱或根本不抑制血凝（HA）。相比之下，HN-IV组抗体强烈抑制HA，但弱中和感染性。n - iv抗体可抑制鸡红细胞或L细胞对SV的吸附，而n - iii抗体几乎不受其抑制。另一方面，将预先吸附SV的HeLa细胞的HN-III抗体在4°C孵育，然后在37°C培养，可以抑制感染，但HN-IV抗体不能有效干扰这种感染。竞争性ELISA结果显示，17种抗f抗体可分为两组（F-I和-II）。然而，被抗体识别的两个抗原位点似乎彼此靠近，因为两组抗体之间存在一定的竞争。7种属于F-II组的抗体中有2种抑制溶血活性，也中和SV的传染性，但其他5种F-II抗体没有。其中一种抗f抗体具有低HI活性，并且在竞争试验中与一种抗hn抗体（HN-IV）竞争。
SummaryMurine monoclonal antibodies against the fusion (F) and hemagglutininneuraminidase (HN) proteins of Sendai virus (SV) were prepared and studied on their antiviral activities, particularly on the neutralization of infectivity. On the analysis with solid phase competitive ELISA, 26 anti-HN antibodies were divided into at least four groups (HN-I, -II, -III and -IV). Antigenic sites recognized by the HN-I, -II, and -III group antibodies topographically separate from each other. Sites recognized by the HN-IV group antibodies overlaps partially with ones recognized by the HN-I, HN-II and -III group antibodies. The antibodies belonging to the HN-III group highly neutralize the infectivity of SV and weakly or not at all inhibit the hemagglutination (HA). In contrast, the HN-IV group antibodies strongly inhibit HA, but weakly neutralize the infectivity. Adsorption of SV to chicken red blood cells or L cells is inhibited by the HN-IV antibodies, but scarcely by the HN-III antibodies. On the other hand, incubation with HN-III antibodies of HeLa cells that have been preadsorbed with SV at 4° C, followed by culture at 37° C, causes inhibition of infection, but the HN-IV antibodies do not effectively interfere with such infection.The competitive ELISA showed that 17 anti-F antibodies were divided into two groups (F-I and -II). Two antigenic sites recognized by the antibodies, however, seem to be near to each other because a certain competition is observed between the antibodies of both groups. Two of the seven antibodies belonging to the F-II group inhibit the hemolysis activity and also neutralize the infectivity of SV, but the other five F-II antibodies do not. One of the anti-F antibodies has a low HI activity, and, in competition tests, competes with one of the anti-HN antibodies (HN-IV).
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单克隆抗体作为新城疫病毒 HN 糖蛋白的功能探针：血凝和神经氨酸酶位点的抗原分离。
DOI：
--
发表时间：
1984
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Iorio,RM;Bratt,MA
通讯作者：
Bratt,MA
描绘副粘病毒糖蛋白上的四个抗原位点，单克隆抗体通过这些位点介导不同的抗病毒活性。
DOI：
--
发表时间：
1982
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Yewdell,J;Gerhard,W
通讯作者：
Gerhard,W
影响因子：
3.7
作者：
MERZ, DC;SCHEID, A;CHOPPIN, PW
通讯作者：
CHOPPIN, PW
仙台病毒的 HN 糖蛋白：参与血凝和神经氨酸酶活性的位点分析。
影响因子：
3.7
作者：
Portner,A
通讯作者：
Portner,A