Discovery of BrATG6 and its potential role in Brassica rapa L. resistance to infection by Plasmodiophora brassicae.
Discovery of BrATG6 and its potential role in Brassica rapa L. resistance to infection by Plasmodiophora brassicae.
复制标题
BrATG6 的发现及其在白菜抗菜根霉感染中的潜在作用。
作者：
Jing Zhang;Wenjie Ge;Hong Chang;X. Xin;R. Ji
由甘蓝根肿菌感染引起的根肿病发生在世界许多地区的十字花科蔬菜作物中，有时会导致产量损失。本研究通过二维电泳在对照和根肿病大白菜（Brassica rapaL.）根部之间发现了差异表达蛋白（0305）。质谱分析表明Bra003466与0305蛋白高度匹配。由于Bra003466的序列与拟南芥等芸苔属植物的ATG6有89%的同源性，故将该基因命名为BrATG6。然而，790 bp 序列与芸苔属数据库中 Bra003466 基因的 cDNA 序列不匹配。本研究克隆了Bra003466的cDNA，发现BrATG6在根部各器官中均高表达。当植物接种 P.芸苔属Woronin中，受感染的大白菜根部BrATG6的表达显着增加。该结果通过逆转录qPCR和原位杂交得到验证。抗病性检测表明，与野生型植物相比，A.拟南芥ATG6缺失突变体更容易被拟南芥感染。芸苔乙烷 WT。这说明BrATG6可能在B．拉帕托P.芸苔属感染。
Clubroot disease, caused byPlasmodiophora brassicaeinfection, occurs in cruciferous vegetable crops in many areas of the world, sometimes leading to yield loss. In this study, a differentially expressed protein (0305), was found between control and clubroot-diseased Chinese cabbage(Brassica rapaL.) roots through two-dimensional electrophoresis. Mass spectrometry analysis showed thatBra003466was highly matched to protein 0305. Because the sequence ofBra003466had 89% percent identity with ATG6 ofArabidopsis thalianaand other Brassica, the gene was named asBrATG6. However, 790 bp sequences were mismatched with the cDNA sequence of theBra003466gene from the Brassica database. In this study, we cloned the cDNA ofBra003466and found theBrATG6was highly expressed in roots among all organs. When plants were inoculated withP. brassicaeWoronin, the expression ofBrATG6was significantly increased in infected roots of Chinese cabbage. This result was verified by reverse transcription-qPCR and in situ hybridization. Examination of disease resistance showed that, compared with wild type plants,A. thaliana ATG6deletion mutants were more easily infected byP. brassicaethan WT. This shows thatBrATG6may play a potential role in the resistance ofB. rapatoP. brassicaeinfection.
影响因子：
3.7
作者：
Hatakeyama K;Suwabe K;Tomita RN;Kato T;Nunome T;Fukuoka H;Matsumoto S
通讯作者：
Matsumoto S
拟南芥质膜与冷驯化相关的动态变化
DOI：
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发表时间：
2006
期刊：
影响因子：
--
作者：
Minami;A.
通讯作者：
A.
影响因子：
4.9
作者：
Inoue, Yuko;Suzuki, Takao;Moriyasu, Yuji
通讯作者：
Moriyasu, Yuji