Identification and characterization of Crr1a, a gene for resistance to clubroot disease (Plasmodiophora brassicae Woronin) in Brassica rapa L.
Identification and characterization of Crr1a, a gene for resistance to clubroot disease (Plasmodiophora brassicae Woronin) in Brassica rapa L.
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DOI：
10.1371/journal.pone.0054745
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Matsumoto S
中科院分区：
文献类型：
--
作者：
Hatakeyama K;Suwabe K;Tomita RN;Kato T;Nunome T;Fukuoka H;Matsumoto S
根肿病是由专性生物营养原生生物芸苔根肿菌 Woronin 引起的，是世界上芸苔属作物最重要的经济病害之一。尽管通过遗传分析和QTL定位已经鉴定出许多根肿病抗性(CR)位点，但针对甘蓝根瘤菌的防御反应的分子机制仍然未知。对最初被鉴定为单个基因座的 Crr1 位点进行精细定位后发现，它包含两个基因位点：Crr1a 和 Crr1b。在这里，我们报告了 Crr1a 的基于图谱的克隆和表征，它赋予白菜对根肿病的抗性。 Crr1aG004 克隆自抗性品系 G004，编码 Toll-Interleukin-1 受体/核苷酸结合位点/富含亮氨酸重复序列 (TIR-NB-LRR) 蛋白，在下胚轴和根的中柱和皮层中表达，在病原体发生继发感染的地方，但不在发生初次感染的根毛中表达。功能获得分析证明，Crr1aG004 单独赋予易感拟南芥和白菜中分离株 Ano-01 的抗性。相比之下，易感等位基因 Crr1aA9709 编码截短的 NB-LRR 蛋白，由于在外显子 1 中插入了一个单独的长末端重复序列 (LTR)，该蛋白缺少一半以上的 TIR 结构域，并且在 LRR 结构域中包含多个替换和插入删除。该研究为进一步分子分析甘蓝疫病防御机制奠定了基础，并将有助于通过标记辅助选择选育甘蓝类蔬菜的抗性品种。数据存储本文报告的序列已存储在GenBank数据库中（登录号AB605024）。
Clubroot disease, caused by the obligate biotrophic protist Plasmodiophora brassicae Woronin, is one of the most economically important diseases of Brassica crops in the world. Although many clubroot resistance (CR) loci have been identified through genetic analysis and QTL mapping, the molecular mechanisms of defense responses against P. brassicae remain unknown. Fine mapping of the Crr1 locus, which was originally identified as a single locus, revealed that it comprises two gene loci, Crr1a and Crr1b. Here we report the map-based cloning and characterization of Crr1a, which confers resistance to clubroot in Brassica rapa. Crr1aG004, cloned from the resistant line G004, encodes a Toll-Interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat (TIR-NB-LRR) protein expressed in the stele and cortex of hypocotyl and roots, where secondary infection of the pathogen occurs, but not in root hairs, where primary infection occurs. Gain-of-function analysis proved that Crr1aG004 alone conferred resistance to isolate Ano-01 in susceptible Arabidopsis and B. rapa. In comparison, the susceptible allele Crr1aA9709 encodes a truncated NB-LRR protein, which lacked more than half of the TIR domain on account of the insertion of a solo-long terminal repeat (LTR) in exon 1 and included several substitutions and insertion-deletions in the LRR domain. This study provides a basis for further molecular analysis of defense mechanisms against P. brassicae and will contribute to the breeding of resistant cultivars of Brassica vegetables by marker-assisted selection. Data deposition The sequence reported in this paper has been deposited in the GenBank database (accession no. AB605024).
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DOI：
10.1073/pnas.97.26.14789
发表时间：
2000-12-19
影响因子：
11.1
作者：
Dinesh-Kumar, SP;Tham, WH;Baker, BJ
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影响因子：
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影响因子：
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