Structural polarity and dynamics of male germline stem cells in the milkweed bug (Oncopeltus fasciatus)
Structural polarity and dynamics of male germline stem cells in the milkweed bug (Oncopeltus fasciatus)
复制标题
马利筋虫（Oncopeltus fasciatus）雄性生殖干细胞的结构极性和动力学
作者：
E. Schmidt;A. Dorn
马利筋虫的雄性生殖系干细胞（GSC）呈现出一种非凡的结构极性，据我们所知，这是任何其他类型的干细胞所无法比拟的。它们由一个核周体和许多从细胞极产生的指向顶端细胞的突起组成，顶端细胞是GSC的拟生态位。这些突起可以穿过相当长的距离，直到它们的末端接触顶端细胞。从孵化到死亡，GSC的预测经历了显着的变化，其顺序已推断出从所有发育阶段的观察。突起形成始于小叶细胞突起，显示细胞膜的小梁向内生长。指状突起是生长和“雕刻”过程的结果。新形成的突起大多只含有游离核糖体，而不含有少量线粒体。一个刻板的降解过程开始在投影终端：轮廓的圆形，往往同心圆，池的粗面内质网出现，变成髓鞘体，而线粒体变得更多。细胞质囊泡化，溶酶体体出现，线粒体肿胀。同时，投射终末被细胞膜的横向向内生长隔开。最后，自噬空泡和髓鞘体填充分离的终末，然后终末破裂。同时，新的突起似乎从GSC的核周体中发芽。这些动力学，这是不同步的GSC之间，表明一种新型的信号交换和干细胞之间的转导和他们的小生境参与调节GSC的不对称与对称分裂。
The male germline stem cells (GSCs) of the milkweed bug present an extraordinary structural polarity that is, to our knowledge, unequalled by any other type of stem cells. They consist of a perikaryon and numerous projections arising from the cell pole directed toward the apical cells, the proposed niche of the GSCs. The projections can traverse a considerable distance until their terminals touch the apical cells. From hatching until death, the GSC projections undergo conspicuous changes, the sequence of which has been deduced from observations of all developmental stages. Projection formation starts from lobular cell protrusions showing trabecular ingrowths of the cell membrane. Finger-like projections result from a process of growth and “carving out”. The newly formed projections contain mostly only free ribosomes other than a few mitochondria. A stereotyped degradation process commences in the projection terminals: profiles of circular, often concentric, cisternae of rough endoplasmic reticulum appear and turn into myelin bodies, whereas mitochondria become more numerous. The cytoplasm vesiculates, lysosomal bodies appear, and mitochondria become swollen. At the same time, the projection terminals are segregated by transverse ingrowths of the cell membrane. Finally, autophagic vacuoles and myelin bodies fill the segregated terminals, which then rupture. Simultaneously, new projections seem to sprout from the perikaryon of the GSCs. These dynamics, which are not synchronized among the GSCs, indicate that a novel type of signal exchange and transduction between the stem cells and their niche is involved in the regulation of asymmetric versus symmetric division of GSCs.
影响因子：
4.6
作者：
E. Matunis;John Tran;Pierre Gönczy;Kim Caldwell;Stephen DiNardo
通讯作者：
E. Matunis;John Tran;Pierre Gönczy;Kim Caldwell;Stephen DiNardo
分化早期生殖细胞存活所需的种系特异性间隙连接蛋白。
DOI：
10.1242/dev.129.10.2529
发表时间：
2002
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Tazuke,SalliI;Schulz,Cordula;Gilboa,Lilach;Fogarty,Mignon;Mahowald,AnthonyP;Guichet,Antoine;Ephrussi,Anne;Wood,CricketG;Lehmann,Ruth;Fuller,MargaretT
通讯作者：
Fuller,MargaretT