Growth inhibitory signalling by TGFbeta is blocked in Ras-transformed intestinal epithelial cells at a post-receptor locus.
Growth inhibitory signalling by TGFbeta is blocked in Ras-transformed intestinal epithelial cells at a post-receptor locus.
复制标题
Ras 转化的肠上皮细胞中 TGFbeta 的生长抑制信号在受体后位点被阻断。
DOI：
10.1016/s0898-6568(03)00010-x
复制
发表时间：
2003
影响因子：
4.8
通讯作者：
Barnard,John
中科院分区：
文献类型：
--
作者：
Jiang,Bo;Zhang,Jin-San;Du,Jianguo;Urrutia,Raul;Barnard,John
The transforming growth factor β (TGFβ) family of growth regulatory peptides plays an important role in the regulation of gastrointestinal epithelial cell homeostasis. Loss of growth inhibitory signalling by TGFβ is common in the context of Ras-transformation and it has been hypothesized that loss of TGFβ receptor II (TGFβRII) expression accounts for the emergence of TGFβ resistance. Here we examine the functional significance of reduced TGFβRII expression in intestinal epithelial cells transformed by oncogenic Ras. TGFβ-induced signalling events downstream of TGFβRII were examined in Ras-transformed RIE-1 cells (RIE-Ras) and compared to the parental RIE-1 line. RIE-Ras cells were resistant to growth inhibition by TGFβ. Neither overexpression of TGFβRII in RIE-Ras cells nor expression of constitutively active TGFβRI restored sensitivity to TGFβ. TGFβ-mediated phosphorylation of Smad2 occurred in TGFβ-resistant RIE-Ras cells, as well as other TGFβ-resistant cells lines (HT-29, SW620) expressing low levels of TGFβRII. Nuclear translocation of Smad2 and Smad4 occurred equally in RIE-Ras and parental RIE cells. The activity of TIEG2, a TGFβ-inducible SP1-like transcription factor, was reduced in RIE-Ras cells, implying that resistance in Ras-transformed RIE cells occurs by a transcriptional mechanism.
登录
查看更多内容
使用甲醇水溶液和乙腈流动相对单体和聚合物 C18 键合相进行热力学比较
DOI：
--
发表时间：
1988
期刊：
影响因子：
--
作者：
A. Stalcup;D. Martire;S. Wise
通讯作者：
S. Wise
液相色谱中平面和非平面多环芳烃在各种化学键固定相上的洗脱行为。
影响因子：
4.1
作者：
K. Jinno;T. Nagoshi;N. Tanaka;M. Okamoto;J. Fetzer;W. R. Biggs
通讯作者：
W. R. Biggs
液相色谱用单体和聚合十八烷基衍生硅胶柱填料碳 13 核磁共振谱的差异
DOI：
--
发表时间：
1987
期刊：
影响因子：
--
作者：
Pankaj Shah;L. Rogers;J. Fetzer
通讯作者：
J. Fetzer
DOI：
--
发表时间：
1986
期刊：
影响因子：
--
作者：
P. Zhu
通讯作者：
P. Zhu
反相液相色谱中键合烷基链长度对亚甲基选择性的影响
影响因子：
1.7
作者：
A. M. Krstulović;H. Colin;A. Tchapla;G. Guiochon
通讯作者：
G. Guiochon