MiR-525-3p enhances the migration and invasion of liver cancer cells by downregulating ZNF395.
MiR-525-3p enhances the migration and invasion of liver cancer cells by downregulating ZNF395.
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DOI：
10.1371/journal.pone.0090867
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
He X
中科院分区：
文献类型：
--
作者：
Pang F;Zha R;Zhao Y;Wang Q;Chen D;Zhang Z;Chen T;Yao M;Gu J;He X
肝癌是癌症相关死亡的主要原因之一。对肝癌更深入的机制了解可能会导致更有效的治疗策略的发展。在我们之前的工作中，我们筛选了646个miRNAs，并确定了11个调控肝癌细胞迁移的miRNAs。目前的研究表明，miR-525-3p在肝癌组织中经常表达上调，miR-525-3p的表达增强可以促进肝癌细胞的迁移和侵袭。锌指蛋白395(ZNF395)是miR-525-3p的直接功能靶基因，在肝癌组织中表达下调。ZNF395高表达可显著抑制肝癌细胞的迁移和侵袭，而下调ZNF395表达可显著增强肝癌细胞的迁移和侵袭能力，提示ZNF395具有抑制肝癌转移的作用。ZNF395表达下调可介导miR-525-3p诱导的肝癌细胞迁移和侵袭。综上所述，miR-525-3p通过直接靶向ZNF395促进肝癌细胞的迁移和侵袭，而miR-525-3p和ZNF395在肝癌的发展过程中都起着重要的作用，这使它们成为潜在的治疗靶点。
Liver cancer is one of leading causes of cancer-related deaths. A deeper mechanistic understanding of liver cancer could lead to the development of more effective therapeutic strategies. In our previous work, we screened 646 miRNAs and identified 11 that regulate liver cancer cell migration. The current study shows that miR-525-3p is frequently up-regulated in liver cancer tissues, and enhanced expression of miR-525-3p can promote liver cancer cell migration and invasion. Zinc finger protein 395 (ZNF395) is the direct functional target gene for miR-525-3p, and it is frequently down-regulated in liver cancer tissues. High expression of ZNF395 can significantly inhibit while knockdown of ZNF395 expression can markedly enhance the migration and invasion of liver cancer cells, suggesting that ZNF395 suppresses metastasis in liver cancer. Down-regulation of ZNF395 can mediate miR-525-3p induced liver cancer cell migration and invasion. In conclusion, miR-525-3p promotes liver cancer cell migration and invasion by directly targeting ZNF395, and the fact that miR-525-3p and ZNF395 both play important roles in liver cancer progression makes them potential therapeutic targets.
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影响因子：
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影响因子：
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