Circular RNA circ0007360 Attenuates Gastric Cancer Progression by Altering the miR-762/IRF7 Axis.
Circular RNA circ0007360 Attenuates Gastric Cancer Progression by Altering the miR-762/IRF7 Axis.
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DOI：
10.3389/fcell.2022.789073
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发表时间：
2022
影响因子：
5.5
通讯作者：
Zhou X
中科院分区：
文献类型：
--
作者：
Xing Y;Chen H;Guo Z;Zhou X
胃癌是全世界的主要健康负担。环状 RNA (circRNA) 是一个新型的非编码 RNA 家族，与包括胃癌在内的多种癌症有关。由于新鉴定的circRNA circ0007360的生物学功能和潜在分子机制尚未得到研究，我们目前的研究重点是circ0007360在胃癌进展中的作用。在将circ0007360定性为细胞质circRNA后，我们揭示了circ0007260对胃癌细胞的存活、迁移、侵袭和干性的抑制作用。随后，miR-762被鉴定为circ0007360的直接靶标microRNA（miRNA），并被证明可作为重要的下游转录本，在circ0007360缺失的情况下实现肿瘤促进作用。此外，我们证明干扰素调节因子7（IRF7）被验证为miR-762的靶基因，可以作为circ0007360的间接靶标来减弱胃癌的进展。此外，体内实验证实了circ0007360耗尽后胃癌细胞生长和干性的增强。总之，我们的结果表明，circ0007360/miR-762/IRF7轴的激活是减缓胃癌进展的新机制。我们的研究揭示了 circ0007360 对胃癌患者的诊断和治疗价值。
Gastric cancer is a major health burden worldwide. Circular RNAs (circRNAs) are a novel family of noncoding RNAs that are involved in multiple types of cancers, including gastric cancer. As biological functions and the underlying molecular mechanisms of the newly identified circRNA circ0007360 have not been investigated, our present study focused on the role of circ0007360 in the progression of gastric cancer. After characterizing circ0007360 as a cytoplasmic circRNA, we revealed the inhibitory effects of circ0007260 on the survival, migration, invasion, and stemness of gastric cancer cells. Subsequently, miR-762 was identified as a direct target microRNA (miRNA) of circ0007360 and was proved to act as a vital downstream transcript to fulfill the tumor-promoting effects in the absence of circ0007360. Furthermore, we demonstrated that interferon regulatory factor 7 (IRF7), which was validated as a target gene of miR-762, serves as an indirect target of circ0007360 to attenuate the progression of gastric cancer. Moreover, in vivo experiments confirmed the potentiation of gastric cancer cell growth and stemness upon depletion of circ0007360. In summary, our results revealed that activation of the circ0007360/miR-762/IRF7 axis is a novel mechanism for the attenuation of gastric cancer progression. Our study unveils the diagnostic and therapeutic values of circ0007360 in patients with gastric cancer.
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