A virus-derived small RNA targets the rice transcription factor ROC1 to induce disease-like symptom

A virus-derived small RNA targets the rice transcription factor ROC1 to induce disease-like symptom
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病毒衍生的小RNA靶向水稻转录因子ROC1以诱导疾病样症状

DOI:
10.1186/s42483-022-00112-6
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发表时间:
2022-03
影响因子:
3.4
通讯作者:
Tong Zhang
Tong Zhang
中科院分区:
农林科学2区
文献类型:
--
作者:
Xiuqin Huang;Feifei Li;Xishan Zhang;Jiahao Chen;Junkai Wang;Jing Wei;Xin Yang;Guohui Zhou;Tong Zhang

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植物中的病毒衍生小干扰RNA(VsiRNAs)可以靶向宿主转录产物来诱导症状的发展。南方水稻黑条矮缩病毒(SRBSDV)是危害水稻最严重的水稻病毒,引起水稻植株严重矮化和根系发育不良,严重威胁水稻生产。在本研究中,我们发现从SRBSDV基因组片段9中产生的vsiRNA(vsiR-S9-18)针对水稻中的转录因子ROC1。SRBSDV的侵染触发了vsiR-S9-18基因的产生,导致水稻ROC1基因表达下调。VsiR-S9-18在水稻原生质体和烟草叶片中的瞬时表达进一步证明了vsiR-S9-18与ROC1的特异性关联。此外,ROC1基因敲除的水稻植株表现为根变短,与SRBSDV感染引起的根生长抑制表型相似。我们认为vsiR-S9-18通过抑制根的伸长而抑制根的伸长,这已经被证明可以调节根的生长。这一发现扩大了我们对vsiRNA在病毒疾病发展中的作用的理解,并有助于开发新的抗病毒方法。
Virus-derived small interfering RNAs (vsiRNAs) in plants can target host transcripts to induce symptom development. Southern rice black-streaked dwarf virus (SRBSDV) is the most damaging rice-infecting virus, which causes severe stunting and poorly developed roots in rice plants, thereby posing a serious threat to rice production. In this study, we show that a vsiRNA (vsiR-S9-18), generated from SRBSDV genome segment 9, targets the transcription factor ROC1 in rice. SRBSDV infection triggered the production of vsiR-S9-18 and resulted in the downregulation ofROC1in rice plants. Transient expression of vsiR-S9-18 in rice protoplasts and tobacco leaves further demonstrated the specific association of vsiR-S9-18 withROC1. Moreover, theROC1-knockout rice plants displayed shortened roots, similar to the phenotype of root growth inhibition caused by SRBSDV infection. We propose that vsiR-S9-18 inhibits root elongation viaROC1, which has been proven to regulate root growth. This finding extends our understanding of the role of vsiRNA in viral disease development and is helpful for the development of new antiviral approaches.
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