Fast acquisition of a polysaccharide fermenting gut microbiome by juvenile green turtles Chelonia mydas after settlement in coastal habitats.
Fast acquisition of a polysaccharide fermenting gut microbiome by juvenile green turtles Chelonia mydas after settlement in coastal habitats.
复制标题
DOI：
10.1186/s40168-018-0454-z
复制
发表时间：
2018-04-10
期刊：
影响因子：
15.5
通讯作者：
Cardona L
中科院分区：
文献类型：
--
作者：
Campos P;Guivernau M;Prenafeta-Boldú FX;Cardona L
四足动物不表达纤维素和半纤维素同化的水解酶，因此，独立收购草食动物需要建立新的内共生关系的四足动物和微生物。绿色海龟（Chelonia mydas）是三种以草食为主的海洋四足动物之一，它们在食用远洋动物数年后才获得草食。我们对来自巴西沿海沃茨的24只年轻野生和圈养绿色海龟的粪便和直肠中存在的微生物群进行了表征，弯曲的甲壳长度从31.1到64.7 cm不等，以检验以下假设：（1）定居后个体发育饮食的转变之后是肠道微生物组的组成和多样性的逐渐变化，（2）热带和亚热带地区绿色龟肠道微生物的组成和多样性存在差异;（3）杂食性食物的消耗改变了绿色龟的肠道微生物。获得了2，186，596个有效细菌16 S rRNA读段的基因组文库，并将这些序列分组为6321个不同的操作分类单位（在97%序列同源性截止值）。结果表明，大多数弯曲背甲长度小于45 cm的幼绿色海龟表现出由拟杆菌门和厚壁菌门的代表共同主导的粪便微生物群，以及后一门中高水平的梭菌科、原单胞菌属、瘤胃球菌科和毛螺菌科。此外，这是在> 45 cm CCL的野生绿色海龟和大多数以大型藻类/鱼类混合饮食为食的任何大小的圈养绿色海龟中发现的唯一微生物群概况。然而，微生物多样性随着海龟的大小而增加，热带地区的海龟比亚热带地区的海龟更高。这些结果表明，少年绿色海龟从巴西沿海沃茨有相同的一般微生物群，无论身体大小和起源，并建议快速收购的多糖发酵肠道微生物群的少年绿色海龟定居到沿海栖息地后。本文的在线版本（10.1186/s40168-018-0454-z）包含补充材料，可供授权用户使用。
Tetrapods do not express hydrolases for cellulose and hemicellulose assimilation, and hence, the independent acquisition of herbivory required the establishment of new endosymbiotic relationships between tetrapods and microbes. Green turtles (Chelonia mydas) are one of the three groups of marine tetrapods with an herbivorous diet and which acquire it after several years consuming pelagic animals. We characterized the microbiota present in the feces and rectum of 24 young wild and captive green turtles from the coastal waters of Brazil, with curved carapace length ranging from 31.1 to 64.7 cm, to test the hypotheses that (1) the ontogenetic dietary shift after settlement is followed by a gradual change in the composition and diversity of the gut microbiome, (2) differences exist between the composition and diversity of the gut microbiome of green turtles from tropical and subtropical regions, and (3) the consumption of omnivorous diets modifies the gut microbiota of green turtles. A genomic library of 2,186,596 valid bacterial 16S rRNA reads was obtained and these sequences were grouped into 6321 different operational taxonomic units (at 97% sequence homology cutoff). The results indicated that most of the juvenile green turtles less than 45 cm of curved carapace length exhibited a fecal microbiota co-dominated by representatives of the phyla Bacteroidetes and Firmicutes and high levels of Clostridiaceae, Prophyromonas, Ruminococaceae, and Lachnospiraceae within the latter phylum. Furthermore, this was the only microbiota profile found in wild green turtles > 45 cm CCL and in most of the captive green turtles of any size feeding on a macroalgae/fish mixed diet. Nevertheless, microbial diversity increased with turtle size and was higher in turtles from tropical than from subtropical regions. These results indicate that juvenile green turtles from the coastal waters of Brazil had the same general microbiota, regardless of body size and origin, and suggest a fast acquisition of a polysaccharide fermenting gut microbiota by juvenile green turtles after settlement into coastal habitats. The online version of this article (10.1186/s40168-018-0454-z) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
5.2
作者：
Abdelrhman KF;Bacci G;Mancusi C;Mengoni A;Serena F;Ugolini A
通讯作者：
Ugolini A
影响因子：
2.6
作者：
BJORNDAL, KA
通讯作者：
BJORNDAL, KA
影响因子：
2.5
作者：
Arthur, Karen E.;Boyle, Michelle C.;Limpus, Colin J.
通讯作者：
Limpus, Colin J.
影响因子：
1.9
作者：
André, J;Gyuris, E;Lawler, IR
通讯作者：
Lawler, IR
影响因子：
1.2
作者：
Eigeland, Karen A.;Lanyon, Janet M.;Klieve, Athol V.
通讯作者：
Klieve, Athol V.