Evaluating the impact of hydroxychloroquine on mouse lymphocyte proliferation and cytokine production in vivo and in vitro.
Evaluating the impact of hydroxychloroquine on mouse lymphocyte proliferation and cytokine production in vivo and in vitro.
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DOI：
10.1016/j.xpro.2021.100517
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发表时间：
2021-06-18
期刊：
影响因子：
--
通讯作者：
Greten TF
中科院分区：
文献类型：
--
作者：
Wabitsch S;McCallen JD;Ma C;Greten TF
免疫调节药物可改变淋巴细胞功能。羟氯喹（HCQ）是许多自身免疫性疾病的处方药，目前正在研究作为一种抗肿瘤自噬抑制剂。在这里，我们描述了一个协议，以评估HCQ对淋巴细胞功能的影响，通过测量在体外和离体增殖和细胞因子的产生。该方案可以提供对HCQ潜在免疫调节作用的见解，并可用于评估其他药物对淋巴细胞功能的影响。有关本方案使用和执行的完整详细信息，请参阅。评估HCQ对小鼠淋巴细胞功能的体外和离体影响评估羟氯喹的免疫调节特性评估其他试剂免疫调节特性的实验框架淋巴细胞功能的研究可在10天内完成免疫调节药物可改变淋巴细胞功能。羟氯喹（HCQ）是许多自身免疫性疾病的处方药，目前正在研究作为一种抗肿瘤自噬抑制剂。在这里，我们描述了一个协议，以评估HCQ对淋巴细胞功能的影响，通过测量在体外和离体增殖和细胞因子的产生。该方案可以提供对HCQ潜在免疫调节作用的见解，并可用于评估其他药物对淋巴细胞功能的影响。
Immunomodulatory drugs can alter lymphocyte function. Hydroxychloroquine (HCQ) is prescribed for many autoimmune diseases and is under investigation as an anti-tumor autophagy inhibitor. Here, we describe a protocol to evaluate the influence of HCQ on lymphocyte function by measuring the in vitro and ex vivo proliferation and cytokine production. The protocol can provide insights into potential immunomodulatory effects of HCQ and can be used for assessing other medications' effects on lymphocyte functions. For complete details on the use and execution of this protocol, please refer to. Evaluate the in vitro and ex vivo effects of HCQ on mouse lymphocyte function Assess the immunomodulatory features of hydroxychloroquine Experimental framework to assess the immunomodulatory features of other reagents Studies of lymphocyte function can be completed within 10 days Immunomodulatory drugs can alter lymphocyte function. Hydroxychloroquine (HCQ) is prescribed for many autoimmune diseases and is under investigation as an anti-tumor autophagy inhibitor. Here, we describe a protocol to evaluate the influence of HCQ on lymphocyte function by measuring the in vitro and ex vivo proliferation and cytokine production. The protocol can provide insights into potential immunomodulatory effects of HCQ and can be used for assessing other medications' effects on lymphocyte functions.
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影响因子：
1.2
作者：
Lim, Jun Feng;Berger, Heidi;Su, I-hsin
通讯作者：
Su, I-hsin
影响因子：
15.9
作者：
Yoneyama, H;Harada, A;Matsushima, K
通讯作者：
Matsushima, K
影响因子：
5.8
作者：
Wabitsch S;McVey JC;Ma C;Ruf B;Kamenyeva O;McCallen JD;Diggs LP;Heinrich B;Greten TF
通讯作者：
Greten TF
影响因子：
4.4
作者：
Spranger, Stefani;Javorovic, Miran;Frankenberger, Bernhard
通讯作者：
Frankenberger, Bernhard