Qing Dai attenuates nonsteroidal anti-inflammatory drug-induced mitochondrial reactive oxygen species in gastrointestinal epithelial cells.
Qing Dai attenuates nonsteroidal anti-inflammatory drug-induced mitochondrial reactive oxygen species in gastrointestinal epithelial cells.
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DOI：
10.3164/jcbn.14-59
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发表时间：
2015-01
影响因子：
2.4
通讯作者：
Hyodo I
中科院分区：
文献类型：
--
作者：
Saito R;Tamura M;Matsui H;Nagano Y;Suzuki H;Kaneko T;Mizokami Y;Hyodo I
非甾体抗炎药（NSAIDs）治疗增加了胃肠道并发症患者的数量。青黛传统上被用于治疗各种炎症性疾病，如溃疡性结肠炎。我们之前报道过清黛通过清除溃疡性结肠炎患者的活性氧（ROS）来抑制炎症。因此，清黛可以减弱ROS的产生，而ROS在nsaid诱导的胃肠道损伤中起重要作用。在这项研究中，我们旨在通过检测细胞损伤、线粒体膜电位和特定荧光指标的ROS生成，阐明清黛是否降低了nsaid处理的胃肠道细胞线粒体ROS生成。我们还使用自旋捕获试剂（CYPMPO或DMPO）对分离的线粒体进行了电子顺磁共振测量。用吲哚美辛和阿司匹林治疗可引起胃肠道细胞损伤和线粒体损伤。在这些条件下，电镜观察线粒体的改变。清戴通过抑制胃肠道细胞中ROS的产生来预防这些并发症。这些结果表明，清黛可减弱非甾体抗炎药诱导的胃肠道上皮细胞线粒体改变产生的ROS。清戴治疗可有效预防非甾体抗炎药引起的胃肠道损伤。
Treatments with nonsteroidal anti-inflammatory drugs (NSAIDs) have increased the number of patients with gastrointestinal complications. Qing Dai has been traditionally used in Chinese herbal medicine for various inflammatory diseases such as ulcerative colitis. We previously reported that Qing Dai suppressed inflammations by scavenging reactive oxygen species (ROS) in ulcerative colitis patients. Thus, Qing Dai can attenuate the production of ROS, which play an important role in NSAID-induced gastrointestinal injuries. In this study, we aimed to elucidate whether Qing Dai decreased mitochondrial ROS production in NSAID-treated gastrointestinal cells by examining cellular injury, mitochondrial membrane potentials, and ROS production with specific fluorescent indicators. We also performed electron paramagnetic resonance measurement in isolated mitochondria with a spin-trapping reagent (CYPMPO or DMPO). Treatments with indomethacin and aspirin induced cellular injury and mitochondrial impairment in the gastrointestinal cells. Under these conditions, mitochondrial alterations were observed on electron microscopy. Qing Dai prevented these complications by suppressing ROS production in gastrointestinal cells. These results indicate that Qing Dai attenuated the ROS production from the NSAID-induced mitochondrial alteration in the gastrointestinal epithelial cells. Qing Dai treatment may be considered effective for the prevention NSAID-induced gastrointestinal injury.
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影响因子：
3.2
作者：
Nagano, Yumiko;Matsui, Hirofumi;Hyodo, Ichinosuke
通讯作者：
Hyodo, Ichinosuke
影响因子：
7.6
作者：
Taha, AS;Angerson, WJ;Blatchford, O
通讯作者：
Blatchford, O
影响因子：
2.4
作者：
Ito, Yoshitsugi;Sasaki, Makoto;Kasugai, Kunio
通讯作者：
Kasugai, Kunio
影响因子：
7.2
作者：
Simon, HU;Haj-Yehia, A;Levi-Schaffer, F
通讯作者：
Levi-Schaffer, F
影响因子：
4.3
作者：
Suzuki, Hideo;Kaneko, Tsuyoshi;Hyodo, Ichinosuke
通讯作者：
Hyodo, Ichinosuke