Codon optimization of the major antigen encoding genes of diverse strains of influenza a virus
Codon optimization of the major antigen encoding genes of diverse strains of influenza a virus
复制标题
甲型流感病毒不同毒株主要抗原编码基因的密码子优化
DOI：
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发表时间：
2011
期刊：
影响因子：
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通讯作者：
M. P. S. Negi
中科院分区：
文献类型：
--
作者：
I. Mani;Vijai Singh;D. Chaudhary;P. Somvanshi;M. P. S. Negi
近年来，全球范围内发生了大量甲型流感疫情和死亡病例，研制有效、足量的疫苗已成为当务之急。疫苗一般是具有免疫原性的蛋白质，由于密码子的偏好性而不能得到足够的表达量，因此有必要在表达宿主中优化其密码子。密码子优化通过提高目的基因的翻译效率来提高蛋白质在生物体中的表达。甲型流感病毒中存在两种表面抗原性糖蛋白，血凝素（HA）和神经氨酸酶（NA）。我们使用来自19株甲型流感病毒的HA和NA基因在大肠杆菌中进行密码子优化。杆菌流感病毒的两个基因显示，与野生型相比，优化DNA中基因的密码子适应指数（CAI）和GC含量显著提高（p <0.01）。优化DNA中HA的CAI和GC分别提高了3.2倍（68.5%）和1.2倍（16.2%），而NA中分别提高了3.3倍（69.7%）和1.2倍（15.8%）。我们的研究结果表明，优化的基因可以用于更好地表达在宿主中没有任何截短的蛋白质，也有助于蛋白质的折叠和功能。这项工作为合成生物学研究提供了新的视角。
A large number of influenza A virus outbreaks and mortality occurred in the world recently, an urgent attention to develop effective and sufficient quantity of vaccines are needed. Vaccines are generally protein with immunogenic properties and are not expressed in sufficient quantity because of the codon bias, so it is necessary to optimize its codon in the expression host. Codon optimization was used to improve the protein expression in living organisms by increasing the translational efficiency of gene of interest. Two surface antigenic glycoproteins, hemagglutinin (HA) and neuraminidase (NA) are present in influenza A viruses. We have used HA and NA genes from 19 strains of influenza A viruses for codon optimization in E. coli. Both genes of the influenza virus show that the codon adaptation index (CAI) and GC content of the genes in optimized DNA were enhanced significantly (p <0.01) as compared to wild type. CAI and GC of HA in optimized DNA was enhanced by 3.2 (68.5%) and 1.2 (16.2%) fold respectively, while in NA it was increased by 3.3 (69.7%) and 1.2 (15.8%) fold respectively. Our finding demonstrates that the optimized genes could be useful for better expression in host without any truncated proteins and also helpful for protein folding and function. This work provides new insight in the synthetic biology research.
影响因子：
14.9
作者：
SHARP, PM;LI, WH
通讯作者：
LI, WH