Overexpression of TC2N is associated with poor prognosis in gastric cancer.
Overexpression of TC2N is associated with poor prognosis in gastric cancer.
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TC2N 过度表达与胃癌不良预后相关
DOI：
10.7150/jca.50653
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发表时间：
2021
影响因子：
3.9
通讯作者：
Peng J
中科院分区：
文献类型：
--
作者：
Xu J;Ou X;Li J;Cai Q;Sun K;Ye J;Peng J
背景：Tac2-N (TC2N) 是一种含有串联 C2 结构域的蛋白质，在不同类型的癌症中充当新型癌基因或抑制基因。然而，TC2N在胃癌（GC）中的表达状况及其意义仍不清楚。本研究旨在阐明GC中TC2N水平的临床病理意义和预后价值。方法：我们使用癌症基因组图谱 (TCGA) 数据库的测序数据，通过 UALCAN 数据库和基因表达谱交互式分析工具 (GEPIA) 分析 GC 中 TC2N 的表达。通过定量实时逆转录聚合酶链反应（RT-PCR）和蛋白质印迹（WB）分析检测12对新鲜GC组织和癌旁非肿瘤组织中TC2N的表达水平。我们中心采用免疫组织化学 (IHC) 染色检测石蜡包埋组织中 TC2N 蛋白的表达。采用体外增殖、迁移和侵袭测定来评估TC2N对胃癌细胞功能的影响。 LinkedOmics 用于识别与 TC2N 相关的基因表达。结果：胃癌组织中TC2N mRNA和蛋白表达量均显着高于正常胃黏膜。与正常胃上皮细胞相比，其在胃癌细胞中也升高。体外实验表明TC2N促进胃癌细胞的增殖、迁移和侵袭。生物信息分析显示TC2N对转录组有广泛的影响，并且与肿瘤相关基因有很强的相互作用。我们还发现TC2N是GC患者长期生存的独立预后因素，其高表达与较差的总生存率和无复发生存率明显相关。结论：我们的结果表明，高水平的TC2N与胃癌患者的不良预后相关，并促进胃癌的发展。因此，TC2N表达可以作为胃癌患者的预后生物标志物。
Background: Tac2-N (TC2N) is a tandem C2 domain-containing protein, acting as a novel oncogene or suppressor in different kinds of cancers. However, the status of TC2N expression and its significance in gastric cancer (GC) is still unclear. The present study is aimed to elucidate the clinicopathological significance and prognostic value of TC2N level in GC. Methods: We used sequencing data from the Cancer Genome Atlas (TCGA) database to analyze TC2N expression in GC by UALCAN database and Gene Expression Profiling Interactive Analysis tools (GEPIA). TC2N expression level in 12 pairs of fresh GC tissues and adjacent nontumorous tissues was detected by quantitative real-time reverse-transcription polymerase chain reaction (RT-PCR) and Western blot (WB) assays. Immunohistochemical (IHC) staining was used to detect TC2N protein expression in Paraffin-embedded tissues in our center. In vitro proliferation, migration and invasion assays were used to evaluate the effect of TC2N on functional capability of gastric cancer cells. LinkedOmics was used to identify gene expressions associated with TC2N. Results: The mRNA and protein expression of TC2N in gastric cancer were both significantly higher than normal gastric mucosa. It was also elevated in gastric cancer cells compared with normal gastric epithelium cell. In vitro assays suggested that TC2N facilitated proliferation, migration and invasion of gastric cancer cells. Bioinformatic analysis showed a widespread impact of TC2N on the transcriptome and a strong interaction with tumor associated genes. We also found that TC2N was an independent prognostic factor for long-term survival in GC patients and its high expression was evidently associated with poor overall survival and recurrence-free survival. Conclusions: Our results show that high level of TC2N correlates with poor prognosis in patients with gastric cancer and promotes the development of gastric cancer. Thus, TC2N expression can serve as a prognostic biomarker for patients with gastric cancer.
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影响因子：
7
作者：
Lee, HK;Hsu, AK;Pavlidis, P
通讯作者：
Pavlidis, P
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影响因子：
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作者：
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影响因子：
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作者：
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影响因子：
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作者：
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Irimura, T
影响因子：
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作者：
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通讯作者：
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