The mitochondrial genome of the pathogenic yeast Candida subhashii: GC-rich linear DNA with a protein covalently attached to the 5' termini.
The mitochondrial genome of the pathogenic yeast Candida subhashii: GC-rich linear DNA with a protein covalently attached to the 5' termini.
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DOI：
10.1099/mic.0.038646-0
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发表时间：
2010-07
期刊：
影响因子：
--
通讯作者：
Nosek J
中科院分区：
文献类型：
--
作者：
Fricova D;Valach M;Farkas Z;Pfeiffer I;Kucsera J;Tomaska L;Nosek J
作为我们旨在对真菌线粒体基因组进行大规模比较分析的倡议的一部分，我们确定了酵母菌苏桥假丝酵母线粒体基因组的完整DNA序列，并发现它具有许多独特的特征。首先，线粒体基因组由长度均匀的线性双链DNA分子（29 795 bp）代表，具有异常高的鸟嘌呤和胞嘧啶残基含量（52.7%）。 第二，编码序列缺少内含子;因此，基因组具有相对紧凑的组织。第三，线性分子的末端由长的反向重复序列组成，并且似乎含有与5′末端核苷酸共价结合的蛋白质。这种结构类似于许多线性病毒和质粒DNA基因组中的端粒，这些基因组被归类为反转子，其中末端蛋白质作为启动DNA合成的特异性引物。最后，虽然C. subhashii包含与其它密切相关的致病性念珠菌属物种基本上相同的基因组，我们鉴定了编码家族B蛋白引发DNA聚合酶的两个同源物和未知蛋白的额外ORF。DNA聚合酶的末端结构和基因让人联想到线性线粒体质粒，表明这种基因组结构可能是由祖先（可能是圆形的）线粒体基因组和反转子样元件之间的偶然重组产生的。
As a part of our initiative aimed at a large-scale comparative analysis of fungal mitochondrial genomes, we determined the complete DNA sequence of the mitochondrial genome of the yeast Candida subhashii and found that it exhibits a number of peculiar features. First, the mitochondrial genome is represented by linear dsDNA molecules of uniform length (29 795 bp), with an unusually high content of guanine and cytosine residues (52.7 %). Second, the coding sequences lack introns; thus, the genome has a relatively compact organization. Third, the termini of the linear molecules consist of long inverted repeats and seem to contain a protein covalently bound to terminal nucleotides at the 5′ ends. This architecture resembles the telomeres in a number of linear viral and plasmid DNA genomes classified as invertrons, in which the terminal proteins serve as specific primers for the initiation of DNA synthesis. Finally, although the mitochondrial genome of C. subhashii contains essentially the same set of genes as other closely related pathogenic Candida species, we identified additional ORFs encoding two homologues of the family B protein-priming DNA polymerases and an unknown protein. The terminal structures and the genes for DNA polymerases are reminiscent of linear mitochondrial plasmids, indicating that this genome architecture might have emerged from fortuitous recombination between an ancestral, presumably circular, mitochondrial genome and an invertron-like element.
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