Tumor ablation using novel photothermal NaxWO3 nanoparticles against breast cancer osteolytic bone metastasis
Tumor ablation using novel photothermal NaxWO3 nanoparticles against breast cancer osteolytic bone metastasis
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使用新型光热NaxWO3纳米粒子对抗乳腺癌溶骨性骨转移的肿瘤消融
DOI：
10.2147/ijn.s217974
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发表时间：
2019-09
影响因子：
8
通讯作者：
Ouyang Zhengxiao
中科院分区：
文献类型：
--
作者：
Jie Shuo;Guo Xiaoning;Ouyang Zhengxiao
背景得益于纳米材料的发展，光热疗法（photothermal therapy，PTT）已被发现是治疗乳腺癌的有效肿瘤消融策略。材料与方法通过简单的热解法合成了新型富氧空位钨青铜纳米粒子（NaxWO 3）。并对其进行了TEM、XRD、UV-vis-NIR、光热转化能力和光热稳定性等测试。200 μg/mL NaxWO 3纳米颗粒作用24和48 h后293 T和4 T1细胞的存活率均在80%以上，证明NaxWO 3具有良好的生物安全性和细胞毒性。建立经皮和胫骨内4 T1乳腺癌模型，采用NaxWO 3（20 mg/kg），功率强度为1.5 W/cm 2的980 nm激光光热治疗。结果在980 nm激光（1 mg/mL）照射下，NaxWO 3纳米粒子的温度在5 min内从25.8°C升高到41.8°C。此外，NaxWO 3纳米颗粒的细胞相容性在生理293 T细胞上被发现，与对乳腺癌4 T1细胞的体外剂量依赖性的显著细胞毒性相反。此外，两种体内乳腺癌模型显示出NaxWO 3纳米颗粒良好的肿瘤消融能力，证明在2周观察期间经皮4 T1肿瘤消除而无复发，以及在体内NaxWO 3 +PTT后胫骨内乳腺癌抑制，骨破坏和肿瘤体积减少。结论首次通过简单的热解工艺制备了一种新型的富氧空位钨青铜纳米粒子（NaxWO 3）。体内外实验均显示合成的NaxWO 3纳米颗粒具有良好的PTT能力和抗乳腺癌溶骨性骨转移的肿瘤消融作用。此外，我们的缺氧NaxWO 3纳米粒子将扩大PTT纳米材料的研究视野。
Backgrounds Profiting from the development of nanomaterials, photothermal therapy (PTT) has been discovered as efficient tumor ablation strategy for breast cancer. Materials and methods Novel oxygen vacancy-rich tungsten bronze nanoparticles (NaxWO3) were synthesized through a simple pyrogenic decomposition process. TEM, XRD, UV-vis-NIR, photothermal conversion ability, and photothermal stability were performed. The viabilities of 293T and 4T1 cells after treating with 200 μg/mL NaxWO3 nanoparticles for 24 or 48 hrs were both above 80%, which proved the good biosafety and cytotoxicity of NaxWO3 in vitro. Two in vivo breast cancer models, namely percutaneous and intratibial 4T1 models were established and NaxWO3 (20 mg/kg) with power intensity of 1.5 W/cm2 980 nm laser photothermal treatment was used in vivo. Results We successfully synthesized ~150 nm NaxWO3 nanoparticles with desirable PTT effects, as evidenced by the temperature increase from 25.8°C to 41.8°C in 5 mins under the irradiation of 980 nm laser (1 mg/mL). Also, cellular compatibility of NaxWO3 nanoparticles was found upon physiologic 293T cells, in contrast with significant cytotoxicity against breast cancer 4T1 cell in vitro dose-dependently. Besides, two in vivo breast cancer models showed the decent tumor ablation ability of NaxWO3 nanoparticles, demonstrating percutaneous 4T1 tumor elimination without recurrence during 2 weeks observation as well as intratibial breast cancer inhibition with decreased bone destruction and tumor volume after NaxWO3+PTT in vivo. Conclusion For the first time, we developed a novel oxygen vacancy-rich tungsten bronze nanoparticles (NaxWO3) through a simple pyrogenic decomposition process for PTT. Both in vitro and in vivo experiments showed the good PTT ability and tumor ablation effects of synthesized NaxWO3 nanoparticles against breast cancer osteolytic bone metastasis. Additionally, our oxygen-deficient NaxWO3 nanoparticles will expand the research horizons of PTT nanomaterials.
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影响因子：
6.2
作者：
Croset, Martine;Goehrig, Delphine;Clezardin, Philippe
通讯作者：
Clezardin, Philippe
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影响因子：
--
作者：
Ouyang Z;Guo X;Chen X;Liu B;Zhang Q;Yin Z;Zhai Z;Qu X;Liu X;Peng D;Shen Y;Liu T;Zhang Q
通讯作者：
Zhang Q
影响因子：
9.3
作者：
Mirrahimi, Mehri;Abed, Ziaeddin;Shakeri-Zadeh, Ali
通讯作者：
Shakeri-Zadeh, Ali
DOI：
10.1093/jnci/djm029
发表时间：
2007-07
期刊：
Journal of the National Cancer Institute
影响因子：
--
作者：
S. Woo;D. Solomon
通讯作者：
S. Woo;D. Solomon
影响因子：
10.8
作者：
Yang, Kai;Zhang, Shuai;Liu, Zhuang
通讯作者：
Liu, Zhuang