Adsorption Characteristics and Enrichment of Emodin from Marine-Derived Aspergillus flavipes HN4-13 Extract by Macroporous Resin XAD-16.
Adsorption Characteristics and Enrichment of Emodin from Marine-Derived Aspergillus flavipes HN4-13 Extract by Macroporous Resin XAD-16.
复制标题
大孔树脂XAD-16对海洋黄曲霉HN4-13提取物大黄素的吸附特性及富集
作者：
大黄素是一种羟基蒽醌衍生物，因其广泛的药理活性而被用作药物已有2000多年的历史。前期研究通过优化海洋黄曲霉HN4-13的发酵条件，实现了大黄素的规模化生产。但发酵液中含有复杂的未知成分，对大黄素的研究产生了不利影响。本文对XAD-16树脂富集黄曲霉HN4-13提取物中大黄素的条件进行了优化，建立了高效、操作简单、成本低、可大制备规模的分离方法。 XAD-16树脂对大黄素的吸附过程符合准二级动力学和Langmuir模型。吸附过程的最佳条件为：大黄素浓度、流速和装载体积分别为0.112 mg/mL、2 BV/h和10 BV。解吸时，用50%乙醇洗脱杂质，用80%乙醇解吸大黄素。经过XAD-16树脂富集后，大黄素含量由1.16%提高到11.48%，一步处理后回收率为75.53%。这些结果证明了使用 XAD-16 树脂富集大黄素的简单吸附-解吸策略的效率。
Emodin, a hydroxyanthraquinone derivative, has been used as medicine for more than 2000 years due to its extensive pharmacological activities. Large-scale production of emodin has been achieved by optimizing the fermentation conditions of marine-derived Aspergillus flavus HN4-13 in a previous study. However, the fermentation broth contained complex unknown components, which adversely affected the study of emodin. Herein, the conditions for the enrichment of emodin from A. flavipes HN4-13 extract using XAD-16 resin were optimized, and a separation method with high efficiency, simple operation, a low cost, and a large preparative scale was established. The adsorption process of emodin on the XAD-16 resin conformed to pseudo-second-order kinetics and Langmuir models. The optimal conditions for the adsorption process were as follows: An emodin concentration, flow rate, and loading volume of 0.112 mg/mL, 2 BV/h, and 10 BV, respectively. For desorption, 50% ethanol was used to elute impurities and 80% ethanol was used to desorb emodin. After enrichment with XAD-16 resin, the emodin content increased from 1.16% to 11.48%, and the recovery rate was 75.53% after one-step treatment. These results demonstrate the efficiency of the simple adsorption–desorption strategy, using the XAD-16 resin for emodin enrichment.
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