Tissue type-specific expression of the dsRNA-binding protein 76 and genome-wide elucidation of its target mRNAs.
Tissue type-specific expression of the dsRNA-binding protein 76 and genome-wide elucidation of its target mRNAs.
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DOI：
10.1371/journal.pone.0011710
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发表时间：
2010-07-23
期刊：
影响因子：
3.7
通讯作者：
Gromeier M
中科院分区：
文献类型：
--
作者：
Neplioueva V;Dobrikova EY;Mukherjee N;Keene JD;Gromeier M
RNA-binding proteins accompany all steps in the life of mRNAs and provide dynamic gene regulatory functions for rapid adjustment to changing extra- or intracellular conditions. The association of RNA-binding proteins with their targets is regulated through changing subcellular distribution, post-translational modification or association with other proteins. We demonstrate that the dsRNA binding protein 76 (DRBP76), synonymous with nuclear factor 90, displays inherently distinct tissue type-specific subcellular distribution in the normal human central nervous system and in malignant brain tumors of glial origin. Altered subcellular localization and isoform distribution in malignant glioma indicate that tumor-specific changes in DRBP76-related gene products and their regulatory functions may contribute to the formation and/or maintenance of these tumors. To identify endogenous mRNA targets of DRBP76, we performed RNA-immunoprecipitation and genome-wide microarray analyses in HEK293 cells, and identified specific classes of transcripts encoding critical functions in cellular metabolism. Our data suggest that physiologic DRBP76 expression, isoform distribution and subcellular localization are profoundly altered upon malignant transformation. Thus, the functional role of DRBP76 in co- or post-transcriptional gene regulation may contribute to the neoplastic phenotype.
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影响因子：
56.9
作者：
Choudhary, Chunaram;Kumar, Chanchal;Mann, Matthias
通讯作者：
Mann, Matthias
迅速发展的人类RNA结合核因子90并显示组织限制的分布。
影响因子：
14.9
作者：
Parrott, Andrew M;Mathews, Michael B
通讯作者：
Mathews, Michael B
DOI：
10.1073/pnas.95.15.8514
发表时间：
1998-07-21
影响因子：
11.1
作者：
Liao, HJ;Kobayashi, R;Mathews, MB
通讯作者：
Mathews, MB
影响因子：
14.9
作者：
Kuwano Y;Pullmann R Jr;Marasa BS;Abdelmohsen K;Lee EK;Yang X;Martindale JL;Zhan M;Gorospe M
通讯作者：
Gorospe M
影响因子：
5.3
作者：
Kuwano, Yuki;Kim, Hyeon Ho;Gorospe, Myriam
通讯作者：
Gorospe, Myriam